NDLI: Fluorescence imaging of cambial zones to study wood formation in Pinus radiata D. Don.

Content Provider	Springer Nature Link
Author	Dickson, Alan Nanayakkara, Bernadette Sellier, Damien Meason, Dean Donaldson, Lloyd Brownlie, Rod
Copyright Year	2016
Abstract	A technique whereby whole mounts of delicate tissues of differentiating xylem are imaged directly by polishing and block-face imaging of embedded microcores. Autofluorescence and image analysis aids identifying the stages of xylogenesis. Stem microcores from fast-growing trees, such as Pinus radiata (D. Don) with wide zones of cambium and differentiating xylem and very wide growth rings, pose a challenge for microscopy, as they are difficult to handle and easily damaged compared to slower growing species. A novel procedure has been developed which captures high-resolution images directly from the block face of large samples embedded in plastic resin without the need for sectioning or staining. Microcores of differentiating xylem of P. radiata growing in the central North Island of New Zealand were embedded in a low viscosity acrylic resin. The surface of the entire resin block was abraded and polished to expose cross sections of the wide zone of wood formation in these fast-growing trees without damage or distortion. Autofluorescence imaging was performed using a confocal laser scanning microscope. This avoided the need for staining and allowed the determination of the beginning of lignification based on lignin autofluorescence. Image analysis was used to determine the widths of: (a) the cambium, cell expansion, and wall-thickening zone (CET) and (b) the wall lignification zone (LT). A fast-growing tree had wider CET and LT zones than a slow-growing tree. This was due to the fast-growing tree producing more tracheids than the slow-growing tree, rather than by the production of larger tracheids.
Starting Page	479
Ending Page	490
Page Count	12
File Format	PDF
ISSN	09311890
Journal	Trees
Volume Number	31
Issue Number	2
e-ISSN	14322285
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-09-28
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Block-face imaging Microcore imaging Autofluorescence Xylogenesis Cambium Forestry Plant Sciences Agriculture Plant Anatomy/Development Plant Pathology Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Ecology Physiology Plant Science Forestry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A model of stem growth and wood formation in Pinus radiata

Concomitant analysis of cambial abscisic acid and cambial growth activity in poplar

Tree growth, cambial phenology, and wood anatomy of limber pine at a Great Basin (USA) mountain observatory

Climatic impacts and drought control of radial growth and seasonal wood formation in Pinus halepensis

Differences in intra-annual wood formation in Picea abies across the treeline ecotone, Giant Mountains, Czech Republic

Variations in the radial growth and wood density components in relation to cambial age in 30-year-old Pinus brutia Ten. at two test sites

Effects of a 20-day-long dry period on cambial and apical meristem growth in Abies balsamea seedlings

Intra-ring checking in Pinus radiata D. Don: the occurrence of cell wall fracture, cell collapse, and lignin distribution

Xylem and phloem phenology in co-occurring conifers exposed to drought

Fluorescence imaging of cambial zones to study wood formation in Pinus radiata D. Don.

Similar Documents

A model of stem growth and wood formation in Pinus radiata

Concomitant analysis of cambial abscisic acid and cambial growth activity in poplar

Tree growth, cambial phenology, and wood anatomy of limber pine at a Great Basin (USA) mountain observatory

Climatic impacts and drought control of radial growth and seasonal wood formation in Pinus halepensis

Differences in intra-annual wood formation in Picea abies across the treeline ecotone, Giant Mountains, Czech Republic

Variations in the radial growth and wood density components in relation to cambial age in 30-year-old Pinus brutia Ten. at two test sites

Effects of a 20-day-long dry period on cambial and apical meristem growth in Abies balsamea seedlings

Intra-ring checking in Pinus radiata D. Don: the occurrence of cell wall fracture, cell collapse, and lignin distribution

Xylem and phloem phenology in co-occurring conifers exposed to drought

Fluorescence imaging of cambial zones to study wood formation in Pinus radiata D. Don.