NDLI: MulSatDB: a first online database for mulberry microsatellites

Content Provider	Springer Nature Link
Author	Ramesh Krishnan, R. Sumathy, R. Bindroo, B. B. Girish Naik, V.
Copyright Year	2014
Abstract	Simple sequence repeat motifs were mined from the genome and EST sequences of Morus notabilis and archived in MulSatDB. Bioinformatics tools were integrated with the database for the analysis of genomic datasets. Mulberry is a crop of economic importance in sericulture, which shapes the lives of millions of rural people among different Eurasian and Latin American countries. Limited availability of genomic resources has constrained the molecular breeding efforts in mulberry, a poorly studied crop. Microsatellite or simple sequence repeat (SSR) has revolutionized the plant breeding and is used in linkage mapping, association studies, diversity, and parentage analysis, etc. Recent availability of mulberry whole genome assembly provided an opportunity for the development of mulberry-specific DNA markers. In this study, we mined a total of 217,312 microsatellites from whole genome and 961 microsatellites from EST sequences of Morus notabilis. Mono-repeats were predominant among both whole genome and EST sequences. The SSR containing EST sequences were functionally annotated, and SSRs mined from whole genome were mapped on chromosomes of the phylogenetically related genus—Fragaria vesca, to aid the selection of markers based on the function and location. All the mined markers were archived in the mulberry microsatellite database (MulSatDB), and the markers can be retrieved based on different criteria like marker location, repeat kind, motif type and size. Primer3plus and CMap tools are integrated with the database to design primers for PCR amplification and to visualize markers on F. vesca chromosomes, respectively. A blast tool is also integrated to collate new markers with the database. MulSatDB is the first and complete destination for mulberry researchers to browse SSR markers, design primers, and locate markers on strawberry chromosomes. MulSatDB is freely accessible at http://btismysore.in/mulsatdb .
Starting Page	1793
Ending Page	1799
Page Count	7
File Format	PDF
ISSN	09311890
Journal	Trees
Volume Number	28
Issue Number	6
e-ISSN	14322285
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-09-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	EST Mulberry Morus Database Microsatellites Marker location SSR Tandem repeats Whole genome Forestry Plant Sciences Agriculture Plant Anatomy/Development Plant Pathology Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Ecology Physiology Plant Science Forestry

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