NDLI: Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology

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Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology

Content Provider	Springer Nature Link
Author	Liu, Bing Lan Tzeng, Yew Min
Copyright Year	1998
Abstract	The effects of cultivation medium compositions including tapioca, fishmeal, CaCO3 and (NH4)2SO4 for the growth of Bacillus thuringiensis YMB 96-1988 were accessed by using response surface methodology (RSM). The two-level (24–1) fractional factorial designs (FFD) which involve two concentrations of each nutrient, and the paths of steepest ascent were effective in searching for the major factors of the bacteria growth. This allows the fitting of a first order linear model to the data. In this study, supplementary CaCO3 showed a negative effect on the spore production based on the first order regression coefficients derived from SAS programme. Subsequently, a 23 central composite design (CCD) was used for allocation of treatment combinations. Preliminary studies showed that tapioca and fishmeal is believed to be the major factors for the growth of B. thuringiensis. Estimated optimum compositions for the production of spores by B. thuringiensis are as follows: tapioca, 5.01%; fishmeal 5.86%; (NH4)2SO4 0.06% and resulted in a maximum spore count of 8.56 × 108/ml was obtained. This value is close to the 8.35 × 108/ml spore density as counted from actual experimental observations.
Starting Page	413
Ending Page	418
Page Count	6
File Format	PDF
ISSN	0178515X
Journal	Bioprocess Engineering
Volume Number	18
Issue Number	6
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-06-22
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology Biotechnology

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