NDLI: Statistical medium optimization for the production of collagenolytic protease by Pseudomonas sp. SUK using response surface methodology

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Statistical medium optimization for the production of collagenolytic protease by Pseudomonas sp. SUK using response surface methodology

Content Provider	Springer Nature Link
Author	Bhagwat, Prashant K. Jhample, Sowmya B. Dandge, Padma B.
Copyright Year	2015
Abstract	Pseudomonas sp. SUK producing an extracellular collagenolytic protease was isolated from soil samples from meat and poultry industrial area based in Kolhapur, India. Response surface methodology was employed for the optimization of different nutritional parameters influencing production of collagenolytic protease by newly isolated Pseudomonas sp. SUK in submerged fermentation. Initial screening of production parameters was performed using Plackett-Burman design and the variables with statistically significant effects on collagenolytic protease production were identified as gelatin, peptone, and K$_{2}$HPO$_{4}$. Further, optimization by response surface methodology (RSM) using Central Composite Design showed optimum production of collagenolytic protease with 12.05 g L$^{−1}$ of gelatin, 12.26 g L$^{−1}$ of peptone and 1.29 g L$^{−1}$ of K$_{2}$HPO$_{4}$. Collagenolytic protease production obtained experimentally has very close agreement with the model prediction value and the model was proven to be adequate. The statistical optimization by response surface methodology upsurges collagenolytic protease yield by 2.9 fold, hence the experimental design is effective towards process optimization. Moreover, ammonium sulphate precipitated, partially purified enzyme has shown to cleave collagen from bovine achilles tendon, which was observed by phase contrast microscopy, and SDS-PAGE. Hence, extracellular collagenolytic protease of Pseudomonas sp. SUK could have considerable potential for industrial as well as medical applications.
Starting Page	520
Ending Page	530
Page Count	11
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	84
Issue Number	4
e-ISSN	16083237
Language	English
Publisher	Pleiades Publishing
Publisher Date	2015-07-26
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pseudomonas sp. SUK collagenolytic protease optimization response surface methodology Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

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