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| Content Provider | Springer Nature Link |
|---|---|
| Author | Guerrero Germán, Patricia Prazeres, Duarte M. F. Guzmán, Roberto Montesis Cisneros, Rosa Ma. Tejeda Mansir, Armando |
| Copyright Year | 2008 |
| Abstract | A new bioprocess using mainly membrane operations to obtain purified plasmid DNA from Escherechia coli ferments was developed. The intermediate recovery and purification of the plasmid DNA in cell lysate was conducted using hollow-fiber tangential filtration and tandem anion-exchange membrane chromatography. The purity of the solutions of plasmid DNA obtained during each process stage was investigated. The results show that more than 97% of RNA in the lysate was removed during the process operations and that the plasmid DNA solution purity increased 28-fold. One of the main characteristics of the developed process is to avoid the use of large quantities of precipitating agents such as salts or alcohols. A better understanding of membrane-based technology for the purification of plasmid DNA from clarified E. coli lysate was developed in this research. The convenience of anion-exchange membranes, configured in ready-to-use devices can further simplify large-scale plasmid purification strategies. |
| Starting Page | 615 |
| Ending Page | 623 |
| Page Count | 9 |
| File Format | |
| ISSN | 16157591 |
| Journal | Bioprocess Engineering |
| Volume Number | 32 |
| Issue Number | 5 |
| e-ISSN | 16157605 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2008-12-13 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Plasmid DNA Membrane chromatography Membrane filtration Ion-exchange Industrial and Production Engineering Food Science Environmental Engineering/Biotechnology Biotechnology Industrial Chemistry/Chemical Engineering |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Biotechnology |
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