NDLI: Antibacterial functionalization of wool fabric via immobilizing lysozymes

Content Provider	Springer Nature Link
Author	Wang, Qiang Fan, Xuerong Hu, Yingjun Yuan, Jiugang Cui, Li Wang, Ping
Copyright Year	2008
Abstract	Greater attention has been given to enzymatic processes of textiles as effective alternatives to conventional chemical treatments because of the non-toxic and eco-friendly characteristics of enzymes as well as the increasingly important requirement for reducing pollution in textile production. A new functionalization method for wool fabrics based on immobilization of lysozymes was investigated in this paper. Wool fabric was first activated with glutaraldehyde, and then employed to covalently immobilize lysozymes. Main immobilization parameters were optimized in terms of the activity of immobilized enzyme. A high activity of the immobilized enzyme was obtained when the fabric was activated at 25 °C for 6 h in a bath containing with 0.2% of glutaraldehyde followed by the immobilization at 4 °C and pH 7.0 for 6 h with 5 g l−1 lysozyme. The scanning electron microscopy and staining tests based on modified Coomassie protein assay (Bradford method) revealed that the lysozyme was fixed covalently on the wool fabric. Wool fabrics immobilizing lysozymes presented a higher ratio of bacteriostasis to Staphylococcus aureus. The durability of antibacterial wool was assessed and the lysozyme immobilized on wool fabric retained ca. 43% of its activity after five cycles of use when taking the activity of the immobilized lysozyme before using as reference.
Starting Page	633
Ending Page	639
Page Count	7
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	32
Issue Number	5
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-12-12
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lysozyme Immobilization Antibacterial functionalization Wool Industrial and Production Engineering Food Science Environmental Engineering/Biotechnology Biotechnology Industrial Chemistry/Chemical Engineering
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

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