NDLI: Factors affecting the activation and inhibition of intracellular enzymes for degradation of 1,2 diamino benzene: kinetics and thermodynamic studies

Content Provider	Springer Nature Link
Copyright Year	2015
Abstract	Citrobacter freundii, the bacterium isolated from marine sediments was capable of degrading 1,2 diamino benzene (DAB), an endocrine disruptor. The mixed intracellular enzymes from C. freundii were extracted and purified. The mixed intracellular enzymes were used for the degradation of DAB and degree of degradation was evaluated in terms of pyruvic acid, the end product, formed. The variables such as effect of pH, temperature and metal ions on the degradation of DAB using mixed intracellular enzymes (MICE) were investigated. The maximum amount of pyruvic acid formed was found to be 569 ± 5 µg with 96 % degradation efficiency at pH 7; temperature 25 °C; zinc nitrate 0.1 mM; and copper sulphate ions 0.15 mM. The stability of MICE at different temperatures and the interaction of MICE with metal ions were confirmed using FT-IR spectroscopy. The formation of pyruvic acid from degradation of DAB followed pseudo-second-order rate kinetics and it was a spontaneous, exothermic process. The activation energy of degradation of DAB by MICE was found to be 82.55 kJ/mol.
Starting Page	2221
Ending Page	2230
Page Count	10
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	38
Issue Number	11
e-ISSN	16157605
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-09-03
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	1,2 Diamino benzene Mixed intracellular enzymes Metal ions Pyruvic acid Citrobacter freundii Biotechnology Industrial and Production Engineering Environmental Engineering/Biotechnology Industrial Chemistry/Chemical Engineering Food Science
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Alleviation of metal and BTEX inhibition on BTEX degradation using PVA-immobilized degrader: kinetic model of BTEX degradation

A novel membrane-integrated fermentation reactor system: application to pyruvic acid production in continuous culture by Torulopsis glabrata

Overcoming the inhibition effects of metal ions in the degradation of benzene and toluene byalcaligenes xylosoxidans y234

High Level Expression of an Acid-Stable Phytase from Citrobacter freundii in Pichia pastoris

A general inhibition kinetics model for ethanol production using a novel carbon source: sodium gluconate

Proteome-based physiological analysis of the metabolically engineered succinic acid producer Mannheimia succiniciproducens LPK7

Kinetics of carbendazim degradation in a horizontal tubular biofilm reactor

Purification and Characterization of Tyrosine Phenol Lyase from Citrobacter freundii

Optimization of the integrated citric acid–methane fermentation process by air stripping and glucoamylase addition

Factors affecting the activation and inhibition of intracellular enzymes for degradation of 1,2 diamino benzene: kinetics and thermodynamic studies

Similar Documents

Alleviation of metal and BTEX inhibition on BTEX degradation using PVA-immobilized degrader: kinetic model of BTEX degradation

A novel membrane-integrated fermentation reactor system: application to pyruvic acid production in continuous culture by Torulopsis glabrata

Overcoming the inhibition effects of metal ions in the degradation of benzene and toluene byalcaligenes xylosoxidans y234

High Level Expression of an Acid-Stable Phytase from Citrobacter freundii in Pichia pastoris

A general inhibition kinetics model for ethanol production using a novel carbon source: sodium gluconate

Proteome-based physiological analysis of the metabolically engineered succinic acid producer Mannheimia succiniciproducens LPK7

Kinetics of carbendazim degradation in a horizontal tubular biofilm reactor

Purification and Characterization of Tyrosine Phenol Lyase from Citrobacter freundii

Optimization of the integrated citric acid–methane fermentation process by air stripping and glucoamylase addition

Factors affecting the activation and inhibition of intracellular enzymes for degradation of 1,2 diamino benzene: kinetics and thermodynamic studies