NDLI: Clustering of Kir4.1 at specialized compartments of the lateral membrane in ependymal cells of rat brain

Content Provider	Springer Nature Link
Author	Fujita, Akikazu Inabe, Atsushi Hibi, Hiroshi Nielsen, Søren Ottersen, Ole Petter Kurachi, Yoshihisa
Copyright Year	2014
Abstract	Brain ependymal cells, which form an epithelial layer covering the cerebral ventricles, have been shown to play a role in the regulation of cerebrospinal and interstitial fluids. The machinery underlying this, however, remains largely unknown. Here, we report the specific localization of an inwardly rectifying K+ channel, Kir4.1, on the ependymal cell membrane suggesting involvement of the channel in this function. Immunohistochemical study with confocal microscopy identified Kir4.1 labeling on the lateral but not apical membrane of ependymal cells. Ultrastructural analysis revealed that Kir4.1-immunogold particles were specifically localized and clustered on adjacent membranes at puncta adherens type junctions, whereas an aquaporin water channel, AQP4, that was also detected on the lateral membrane only occurred at components other than adherens junctions. Therefore, in ependymal cells, Kir4.1 and AQP4 are partitioned into distinct membrane compartments that might respectively transport either K+ or water. Kir4.1 was also expressed in a specialized form of ependymal cell, namely the tanycyte, being abundant in tanycyte processes wrapping neuropils and blood vessels. These specific localizations suggest that Kir4.1 mediates intercellular K+ exchange between ependymal cells and also K+-buffering transport via tanycytes that can interconnect neurons and vessels/ventricles. We propose that ependymal cells and tanycytes differentially operate Kir4.1 and AQP4 actively to control the property of fluids at local areas in the brain.
Starting Page	627
Ending Page	634
Page Count	8
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	359
Issue Number	2
e-ISSN	14320878
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-11-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	K+ channel Aquaporin K+-buffering Immunohistochemistry Immuno-electron microscopy Adherens junction Rat (Wistar, male) Human Genetics Proteomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

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