NDLI: Presence and localization of aquaporin-6 in rat parotid acinar cells

Content Provider	Springer Nature Link
Author	Matsuki Fukushima, Miwako Hashimoto, Sadamitsu Shimo, Masaki Satoh, Keitaro Fujita Yoshigaki, Junko Sugiya, Hiroshi
Copyright Year	2007
Abstract	Aquaporins (AQPs) are integral membrane proteins that function as channels for the transfer of water and small solutes across membranes. In mammalian cells, 13 isoforms (AQP0-12) have been identified, and these exhibit unique patterns of expression in various cell types and tissues. Among these isoforms, AQP6 is considered to function not as water channel, but as an anion channel. We investigated the presence and localization of AQP6 in rat parotid acinar cells. AQP6 mRNA was detected in these cells by using reverse transcription/polymerase chain reaction, and Western blotting analysis identified a protein band that reacted with an anti-AQP6 antibody in the membrane fraction and secretory granule membrane. In order to localize AQP6, we used the anti-AQP6 antibody for histological immunodetection. Under confocal microscopy, we observed positive immunoreactions near the tight junctions of parotid acinar cells. Immunolabeling of ultrathin cryosections detected AQP6 near tight junctions and around secretory granule membranes. Immunoelectron microscopy confirmed the presence of AQP6 in the membranes of isolated secretory granules. These results suggest that AQP6 participates in water and anion transport in plasma membranes near tight junctions and secretory granule membranes in rat parotid acinar cells.
Starting Page	73
Ending Page	80
Page Count	8
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	332
Issue Number	1
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-01-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aquaporin-6 Anion channel Apical membrane Secretory granules Parotid Rat (Sprague Dawley, male) Molecular Medicine Proteomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A primary culture of parotid acinar cells retaining capacity for agonists-induced amylase secretion and generation of new secretory granules

Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells

Role of Aquaporin-6 in Rat Parotid Secretory Granules

Aquaporin-1 in blood vessels of rat circumventricular organs

Postnatal expression and denervation induced up-regulation of aquaporin-5 protein in rat sweat gland

Galanin in adrenocorticotropic hormone cells is decreased by castration

Ultrastructural immunogold localization of nitric oxide synthase isoforms in rat and human eosinophils

Expression and localization of TRPC proteins in rat ventricular myocytes at various developmental stages

Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle

Presence and localization of aquaporin-6 in rat parotid acinar cells

Similar Documents

A primary culture of parotid acinar cells retaining capacity for agonists-induced amylase secretion and generation of new secretory granules

Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells

Role of Aquaporin-6 in Rat Parotid Secretory Granules

Aquaporin-1 in blood vessels of rat circumventricular organs

Postnatal expression and denervation induced up-regulation of aquaporin-5 protein in rat sweat gland

Galanin in adrenocorticotropic hormone cells is decreased by castration

Ultrastructural immunogold localization of nitric oxide synthase isoforms in rat and human eosinophils

Expression and localization of TRPC proteins in rat ventricular myocytes at various developmental stages

Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle

Presence and localization of aquaporin-6 in rat parotid acinar cells