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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhang, Jun Bai, Xiaozhi Zhao, Bin Wang, Yunchuan Su, Linlin Chang, Peng Wang, Xujie Han, Shichao Gao, Jianxin Hu, Xiaolong Hu, Dahai Liu, Xiaoyan |
| Copyright Year | 2015 |
| Abstract | Autologous adipose-derived stem cells (ADSCs) can protect fat grafts in cell-assisted lipotransfer (CAL). However, diabetes alters the intrinsic properties of ADSCs and impairs their function so that they lack these protective effects. We investigate whether allogeneic ADSCs from healthy donors could protect fat grafts in immunocompetent diabetic rats. Syngeniec adipose tissues and ADSCs were derived from diabetic Lewis (LEW) rats, whereas allogeneic ADSCs were from healthy brown-Norway rats. A grafted mixture containing 0.7 ml granule fat and 0.3 ml 6 × 106 allogeneic/syngeneic ADSCs was injected subcutaneously on the skulls of diabetic LEW rats. Fat samples were harvested to evaluate the levels of injury and vascularization as shown by perilipin A, CD34 and VEGF at 14 days. The immune response was evaluated with a lymphocytotoxicity test and the CD4/CD8 ratio in peripheral blood at 14 days. The volume retention of fat grafts was measured at 3 months. Healthy allogeneic ADSCs increased the expression levels of perilipin A, CD34 and VEGF at 14 days. The volume retention of fat grafts was improved by allogeneic ADSCs at 3 months. ADSCs were demonstrated to have low immunogenicity by the lymphocyte proliferation test and immunophenotype including MHC and co-stimulatory markers. The lymphocytotoxicity test and CD4/CD8 ratio indicated no obvious immune response elicited by allogeneic ADSCs. Thus, healthy allogeneic ADSCs can promote the survival of fat grafts in this immunocompetent diabetic rat model, with little or no obvious immune rejection. |
| Starting Page | 357 |
| Ending Page | 367 |
| Page Count | 11 |
| File Format | |
| ISSN | 0302766X |
| Journal | Cell and Tissue Research |
| Volume Number | 364 |
| Issue Number | 2 |
| e-ISSN | 14320878 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2015-12-14 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Autologous adipose-derived stem cells Allogeneic stem cell Cell transplantation Fat graft Immune response Human Genetics Proteomics Molecular Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Histology Pathology and Forensic Medicine |
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