NDLI: Tob1 is expressed in developing and adult gonads and is associated with the P-body marker, Dcp2

Content Provider	Springer Nature Link
Author	Shapouri, Farnaz Saeidi, Shaghayegh Iongh, Robb U. Casagranda, Franca Western, Patrick S. McLaughlin, Eileen A. Sutherland, Jessie M. Hime, Gary R. Familari, Mary
Copyright Year	2015
Abstract	Tob1 is a member of the BTG/TOB family of proteins with established antiproliferative function. In Danio rerio and Xenopus laevis, the Tob1 gene is expressed from the one-cell stage through to early gastrula stages, followed in later development by discrete expression in many tissues including the notochord and somites. In both mouse and human, Tob1 is expressed in many adult tissues including the testis and ovary; however, the specific cell types are unknown. We examine Tob1 gene expression in mouse in developing germ cells and in sorted male germ cells (gonocytes, spermatogonia, pachytene spermatocytes and round spermatids) by reverse transcription and droplet digital polymerase chain reaction (RT-ddPCR) and in adult ovary and testis by immunofluorescence with anti-Tob1 protein staining. By RT-ddPCR, Tob1 expression was low in developing male germ cells but was highly expressed in round spermatids. In developing female germ cells undergoing entry into meiosis, it increased 10-fold. Tob1 was also highly expressed in round spermatids and in oocytes in all stages of folliculogenesis. Notably, a marker for P-bodies, Dcp-2, was also highly expressed in round spermatids and all oocyte stages examined. The cytoplasmic presence of Tob1 protein in round spermatids and oocytes and the association of Tob1 protein with Dcp2 in both cell types suggest that Tob1 protein plays a role in post-transcriptional mechanisms.
Starting Page	443
Ending Page	451
Page Count	9
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	364
Issue Number	2
e-ISSN	14320878
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-12-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dcp2 Germ cells P-bodies Tob1 Droplet digital PCR Human Genetics Proteomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

BFGF signaling-mediated reprogramming of porcine primordial germ cells

Prominin-1/CD133, a neural and hematopoietic stem cell marker, is expressed in adult human differentiated cells and certain types of kidney cancer

VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration

Cochlear efferents in developing adult and pathological conditions

Bone marrow as a source of stem cells and germ cells? Perspectives for transplantation

Formation and cultivation of medaka primordial germ cells

RNF4 Is a Growth Inhibitor Expressed in Germ Cells but Not in Human Testicular Tumors

INHIBIN BODIES: A NEW MARKER FOR IMMATURE SERTOLI CELLS

RNF4 is a growth inhibitor expressed in germ cells but not in human testicular tumors.

Tob1 is expressed in developing and adult gonads and is associated with the P-body marker, Dcp2

Similar Documents

BFGF signaling-mediated reprogramming of porcine primordial germ cells

Prominin-1/CD133, a neural and hematopoietic stem cell marker, is expressed in adult human differentiated cells and certain types of kidney cancer

VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration

Cochlear efferents in developing adult and pathological conditions

Bone marrow as a source of stem cells and germ cells? Perspectives for transplantation

Formation and cultivation of medaka primordial germ cells

RNF4 Is a Growth Inhibitor Expressed in Germ Cells but Not in Human Testicular Tumors

INHIBIN BODIES: A NEW MARKER FOR IMMATURE SERTOLI CELLS

RNF4 is a growth inhibitor expressed in germ cells but not in human testicular tumors.

Tob1 is expressed in developing and adult gonads and is associated with the P-body marker, Dcp2