NDLI: Extracellular syntaxin4 triggers the differentiation program in teratocarcinoma F9 cells that impacts cell adhesion properties

Content Provider	Springer Nature Link
Author	Hagiwara, Natsumi Kado, Nanako Miyazaki, Takafumi Maekubo, Kenji Hirai, Yohei
Copyright Year	2013
Abstract	The proteins in the syntaxin family are known to mediate fusion of cytoplasmic vesicles to the target membrane, yet subpopulations of certain syntaxins, including syntaxin4, translocate across the cell membrane in response to external stimuli. Here, we show that extracellularly presented syntaxin4 impacts cell behavior and differentiation in teratocarcinoma F9 cells. While undifferentiated F9 cells extruded a small subpopulation of extracellular syntaxin4 at the lateral cell membrane, the induction of differentiation with all-trans retinoic acid (RA) abolished this localized expression pattern. We found that the cells that were stimulated in a non-directional fashion by extracellular syntaxin4 displayed a flattened shape and retained a substrate-bound morphology even under a long-term, serum-starved cultivation. Such a cellular response was also elicited by a circular peptide composed of the potential functional core of syntaxin4 (AIEPQK; amino acid residues 103∼108) (ST4n1). While the proliferation and metabolism were not affected in these cells, cell–cell interaction became weakened and the expression of vinculin, a regulator of both intercellular and cell-substrate adhesion molecules, was altered. We also found that the expressions of several differentiation markers were up-regulated in cells stimulated with extracellular syntaxin4 and that syntaxin3, another family member, was most prominent. Intriguingly, forced expression of syntaxin3 induced the spread morphology in F9 cells, indicating that syntaxin3 partly mediates the function of extracellular syntaxin4. These results demonstrate the involvement of a non-directional stimulation of extracellular syntaxin4 in the functional and morphological differentiation of F9 cells.
Starting Page	581
Ending Page	591
Page Count	11
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	354
Issue Number	2
e-ISSN	14320878
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-07-25
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Extracellular localization Syntaxin4 F9 Differentiation Epimorphin Human Genetics Proteomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Differentiation of human adipose-derived stem cells towards cardiomyocytes is facilitated by laminin

Interstitial cell migration: integrin-dependent and alternative adhesion mechanisms

Testicular acid phosphatase induces odontoblast differentiation and mineralization

Localization of growth hormone receptors in rat and human thyroid cells

Cell interactions with the extracellular matrix

Pluripotency of embryonic stem cells

Octacalcium phosphate suppresses chondrogenic differentiation of ATDC5 cells

Expression of adhesion molecules during normal pregnancy

Physical regulation of stem cells differentiation into teno-lineage: current strategies and future direction

Extracellular syntaxin4 triggers the differentiation program in teratocarcinoma F9 cells that impacts cell adhesion properties

Similar Documents

Differentiation of human adipose-derived stem cells towards cardiomyocytes is facilitated by laminin

Interstitial cell migration: integrin-dependent and alternative adhesion mechanisms

Testicular acid phosphatase induces odontoblast differentiation and mineralization

Localization of growth hormone receptors in rat and human thyroid cells

Cell interactions with the extracellular matrix

Pluripotency of embryonic stem cells

Octacalcium phosphate suppresses chondrogenic differentiation of ATDC5 cells

Expression of adhesion molecules during normal pregnancy

Physical regulation of stem cells differentiation into teno-lineage: current strategies and future direction

Extracellular syntaxin4 triggers the differentiation program in teratocarcinoma F9 cells that impacts cell adhesion properties