NDLI: Tenascin-C is expressed by human glioma in vivo and shows a strong association with tumor blood vessels

Content Provider	Springer Nature Link
Author	Brösicke, Nicole Landeghem, Frank K. H. Scheffler, Björn Faissner, Andreas
Copyright Year	2013
Abstract	The extracellular matrix (ECM) protein tenascin-C (TN-C) is upregulated within glioma tissues and cultured glioma cell lines. TN-C possesses a multi-modular structure and a variety of functional properties have been reported for its domains. We describe five novel monoclonal antibodies identifying different domains of TN-C. The epitopes for these antibodies were investigated by using recombinantly expressed fibronectin type III domains of TN-C. The biological effects of TN-C fragments on glioma cell proliferation and adhesion were analyzed. The expression pattern of TN-C in human glioma tissue sections and in glioma cell lines was studied with the novel library of monoclonal antibodies. The immunocytochemical analyses of the established human glioma cell lines U-251-MG, U-373-MG and U-87-MG revealed distinct staining patterns for each antibody. Robust expression of TN-C was found within the tumor mass of surgery specimens from glioblastoma. In many cases, the expression of this ECM molecule was clearly associated with blood vessels, particularly with microvessels. Three of the new antibodies highlighted individual TN-C-expressing single cells in glioma tissues. The effect of TN-C domains on glioma cells was examined by a BrdU-proliferation assay and an adhesion assay. Short fragments of constitutively expressed TN-C-domains did not exert significant effects on the proliferation of glioma cells, whereas the intact molecule increased cell division rates. In contrast, the long fragment TNfnALL containing all of the FNIII domains of TN-C decreased proliferation. Additionally, we found strong differences between the adhesion-influencing properties of the recombinant fragments on glioma cells.
Starting Page	409
Ending Page	430
Page Count	22
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	354
Issue Number	2
e-ISSN	14320878
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-08-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Extracellular matrix FNIII domains Monoclonal antibodies Glioblastoma Cancer Proliferation Biopsy Human Human Genetics Proteomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effects of tenascin-W on osteoblasts in vitro

Distribution pattern of Tenascin-C in glioblastoma: Correlation with angiogenesis and tumor cell proliferation

Glioblastoma stem cells

Human monoclonal antibodies to domain C of tenascin-C selectively target solid tumors in vivo

Cell interactions with the extracellular matrix

A novel culture platform for fast proliferation of human annulus fibrosus cells

ATP promotes extracellular matrix biosynthesis of intervertebral disc cells

Extracellular matrix genes as hypoxia-inducible targets

The distribution of extracellular matrix proteins and CD44S expression in human astrocytomas

Tenascin-C is expressed by human glioma in vivo and shows a strong association with tumor blood vessels

Similar Documents

Effects of tenascin-W on osteoblasts in vitro

Distribution pattern of Tenascin-C in glioblastoma: Correlation with angiogenesis and tumor cell proliferation

Glioblastoma stem cells

Human monoclonal antibodies to domain C of tenascin-C selectively target solid tumors in vivo

Cell interactions with the extracellular matrix

A novel culture platform for fast proliferation of human annulus fibrosus cells

ATP promotes extracellular matrix biosynthesis of intervertebral disc cells

Extracellular matrix genes as hypoxia-inducible targets

The distribution of extracellular matrix proteins and CD44S expression in human astrocytomas

Tenascin-C is expressed by human glioma in vivo and shows a strong association with tumor blood vessels