NDLI: The challenges involved in elucidating the molecular basis of sperm–egg recognition in mammals and approaches to overcome them

Content Provider	Springer Nature Link
Author	Wright, Gavin J. Bianchi, Enrica
Copyright Year	2015
Abstract	Sexual reproduction is used by many different organisms to create a new generation of genetically distinct progeny. Cells originating from separate sexes or mating types segregate their genetic material into haploid gametes which must then recognize and fuse with each other in a process known as fertilization to form a diploid zygote. Despite the central importance of fertilization, we know remarkably little about the molecular mechanisms that are involved in how gametes recognize each other, particularly in mammals, although the proteins that are displayed on their surfaces are almost certainly involved. This paucity of knowledge is largely due to both the unique biological properties of mammalian gametes (sperm and egg) which make them experimentally difficult to manipulate, and the technical challenges of identifying interactions between membrane-embedded cell surface receptor proteins. In this review, we will discuss our current knowledge of animal gamete recognition, highlighting where important contributions to our understanding were made, why particular model systems were helpful, and why progress in mammals has been particularly challenging. We discuss how the development of mammalian in vitro fertilization and targeted gene disruption in mice were important technological advances that triggered progress. We argue that approaches employed to discover novel interactions between cell surface gamete recognition proteins should account for the unusual biochemical properties of membrane proteins and the typically highly transient nature of their interactions. Finally, we describe how these principles were applied to identify Juno as the egg receptor for sperm Izumo1, an interaction that is essential for mammalian fertilization.
Starting Page	227
Ending Page	235
Page Count	9
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	363
Issue Number	1
e-ISSN	14320878
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-07-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Egg Fertilization Juno Izumo1 Membrane protein Human Genetics Proteomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

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The challenges involved in elucidating the molecular basis of sperm–egg recognition in mammals and approaches to overcome them

The challenges involved in elucidating the molecular basis of sperm–egg recognition in mammals and approaches to overcome them

Cross-species fertilization: the hamster egg receptor, Juno, binds the human sperm ligand, Izumo1.

Mammalian zona pellucida glycoproteins: structure and function during fertilization

The composition, protein genesis and significance of the inner acrosomal membrane of eutherian sperm

Structure of IZUMO1-JUNO Reveals Sperm-Oocyte Recognition during Mammalian Fertilization

Novel insights into the molecular mechanism of sperm–egg fusion via IZUMO1

Signal transduction in mammalian oocytes during fertilization

Tetraspanin family protein CD9 in the mouse sperm: unique localization, appearance, behavior and fate during fertilization

The challenges involved in elucidating the molecular basis of sperm–egg recognition in mammals and approaches to overcome them

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The challenges involved in elucidating the molecular basis of sperm–egg recognition in mammals and approaches to overcome them

The challenges involved in elucidating the molecular basis of sperm–egg recognition in mammals and approaches to overcome them

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The challenges involved in elucidating the molecular basis of sperm–egg recognition in mammals and approaches to overcome them