NDLI: Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta

Content Provider	Springer Nature Link
Author	Belkacemi, Louiza Gariépy, Gilles Mounier, Catherine Simoneau, Lucie Lafond, Julie
Copyright Year	2003
Abstract	During pregnancy, the calcium (Ca2+) transport machinery of the placenta is solely responsible for the nutrient supply to the developing fetus, where active Ca2+ transport occurs from the mother to the fetus. As part of a larger study to determine the role of Ca2+ in placental transport in vivo, we questioned whether calbindin-D9k (CaBP9k), which is mainly expressed in duodenum, uterus, and placenta of several mammals, is present in cytotrophoblast cells and syncytiotrophoblasts of human term placenta. We were interested in this protein because of its potential importance in serving as an indicator of Ca2+ availability and utilization in the placenta. Here, we demonstrated that CaBP9k transcript is present in both cell types, with a lower expression in cytotrophoblast cells as compared to syncytiotrophoblasts. Moreover, we showed by immunochemistry that CaBP9k protein was present in cytotrophoblast and syncytiotrophoblast placental tissue sections as well as in cultured cells. The occurrence of CaBP9k protein in trophoblast cells was further confirmed by Western blot analysis. Thus, these results indicate for the first time that CaBP9k is unequivocally expressed by trophoblast cells from human term placenta.
Starting Page	107
Ending Page	117
Page Count	11
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	315
Issue Number	1
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-10-28
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Placenta CaBP9k Cytotrophoblast cells Syncytiotrophoblasts Human
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

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