NDLI: Adhesive interactions between CD34+-derived dendritic cell precursors and dermal microvascular endothelial cells studied by scanning electron microscopy

Content Provider	Springer Nature Link
Author	Nguyen, Van Anh Kirchmair, Martin Fürhapter, Christina Romani, Nikolaus Sepp, rbert
Copyright Year	2003
Abstract	Dendritic cells are migratory cells. Before they extravasate from the circulation into the skin across capillary blood vessel walls, they have to interact with endothelial cells. Using a fluorimetric adhesion assay, we have recently shown that CD34+-derived dendritic cell precursors are able to bind to resting and stimulated dermal microvascular endothelial cells. In the present study, we attempted to visualize this process at an ultrastructural level. CD34+ progenitor cells were purified from human cord blood samples by means of immunomagnetic beads, and dendritic cells were generated by culture in the presence of GM-CSF, TNF-α and hSCF for 5 days. Immature CD83− CD86low dendritic cells were added to human dermal microvascular endothelial cells grown to confluence on membrane chambers. After 2 h, unbound dendritic cell precursors were removed, and bound cells were prepared for routine scanning electron microscopy. We found that (1) dendritic cell precursors firmly adhere to microvascular endothelial cells, enveloping them with their surface processes; (2) dendritic cell precursors are extremely deformable as they squeeze through the dense network of microvascular endothelial cells; (3) microvascular endothelial cells form, in part, a multi-layered network rather than the typical cobblestone pattern as seen by phase-contrast microscopy. The morphology of dendritic cell precursors and of human dermal microvascular endothelial cells was examined here, for the first time, by scanning electron microscopy. These data further emphasize that CD34+-derived dendritic cells efficiently adhere to dermal microvascular endothelial cells.
Starting Page	139
Ending Page	143
Page Count	5
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	315
Issue Number	1
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-10-22
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dendritic cells Dendritic cell precursor Dermal microvascular endothelial cell Interaction Scanning electron microscopy CD34+ progenitor cells Human
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

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