NDLI: Localisation of keratin K78 in the basal layer and first suprabasal layers of stratified epithelia completes expression catalogue of type II keratins and provides new insights into sequential keratin expression

Content Provider	Springer Nature Link
Author	Langbein, Lutz Eckhart, Leopold Fischer, Heinz Rogers, Michael A. Praetzel Wunder, Silke Parry, David A. D. Kittstein, Walter Schweizer, Juergen
Copyright Year	2015
Abstract	Among the 26 human type II keratins, K78 is the only one that has not yet been explored with regard to its expression characteristics. Here, we show that, at both the transcriptional and translational levels, K78 is strongly expressed in the basal and parabasal cell layers with decreasing intensity in the lower suprabasal cells of keratinising and non-keratinising squamous epithelia and keratinocyte cultures. The same pattern has been detected at the transcriptional level in the corresponding mouse epithelia. Murine K78 protein, which contains an extraordinary large extension of its tail domain, which is unique among all known keratins, is not detectable by the antibody used. Concomitant studies in human epithelia have confirmed K78 co-expression with the classical basal keratins K5 and K14. Similarly, K78 co-expression with the differentiation-related type I keratins K10 (epidermis) and K13 (non-keratinising epithelia) occurs in the parabasal cell layer, whereas that of the corresponding type II keratins K1 (epidermis) and K4 (non-keratinising epithelia) unequivocally starts subsequent to the respective type I keratins. Our data concerning K78 expression modify the classical concept of keratin pair K5/K14 representing the basal compartment and keratin pairs K1/K10 or K4/K13 defining the differentiating compartment of stratified epithelia. Moreover, the K78 expression pattern and the decoupled K1/K10 and K4/K13 expression define the existence of a hitherto unperceived early differentiation stage in the parabasal layer characterized by K78/K10 or K78/K13 expression.
Starting Page	735
Ending Page	750
Page Count	16
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	363
Issue Number	3
e-ISSN	14320878
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-09-04
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Intermediate filaments Cytoskeleton Differentiation Gene expression Epidermis Human Mouse Human Genetics Proteomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mouse differentiation-specific keratins 1 and 10 require a preexisting keratin scaffold to form a filament network

Cytoskeleton in motion: the dynamics of keratin intermediate filaments in epithelia

Cytoskeleton in motion: the dynamics of keratin intermediate filaments in epithelia

Differential extraction of keratin subunits and filaments from normal human epidermis (1990).

New facets of keratin K77: interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice

Identification of keratins and analysis of their expression in carp and goldfish: comparison with the zebrafish and trout keratin catalog

Functional Differences between Keratins of Stratified and Simple Epithelia

The role of keratin subfamilies and keratin pairs in the formation of human epidermal intermediate filaments (1986)

Differential extraction of keratin subunits and filaments from normal human epidermis.

Localisation of keratin K78 in the basal layer and first suprabasal layers of stratified epithelia completes expression catalogue of type II keratins and provides new insights into sequential keratin expression

Similar Documents

Mouse differentiation-specific keratins 1 and 10 require a preexisting keratin scaffold to form a filament network

Cytoskeleton in motion: the dynamics of keratin intermediate filaments in epithelia

Cytoskeleton in motion: the dynamics of keratin intermediate filaments in epithelia

Differential extraction of keratin subunits and filaments from normal human epidermis (1990).

New facets of keratin K77: interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice

Identification of keratins and analysis of their expression in carp and goldfish: comparison with the zebrafish and trout keratin catalog

Functional Differences between Keratins of Stratified and Simple Epithelia

The role of keratin subfamilies and keratin pairs in the formation of human epidermal intermediate filaments (1986)

Differential extraction of keratin subunits and filaments from normal human epidermis.

Localisation of keratin K78 in the basal layer and first suprabasal layers of stratified epithelia completes expression catalogue of type II keratins and provides new insights into sequential keratin expression