NDLI: Identification of keratins and analysis of their expression in carp and goldfish: comparison with the zebrafish and trout keratin catalog

Content Provider	Springer Nature Link
Author	García, Dana M. Bauer, Hermann Dietz, Thomas Schubert, Thomas Markl, Jürgen Schaffeld, Michael
Copyright Year	2005
Abstract	With more than 50 genes in human, keratins make up a large gene family, but the evolutionary pressure leading to their diversity remains largely unclear. Nevertheless, this diversity offers a means to examine the evolutionary relationships among organisms that express keratins. Here, we report the analysis of keratins expressed in two cyprinid fishes, goldfish and carp, by two-dimensional polyacrylamide gel electrophoresis, complementary keratin blot binding assay, and immunoblotting. We further explore the expression of keratins by immunofluorescence microscopy. Comparison is made with the keratin expression and catalogs of zebrafish and rainbow trout. The keratins among these fishes exhibit a similar range of molecular weights and isoelectric points, with a similar overall pattern on two-dimensional gels. In addition, immunofluorescence microscopy studies of goldfish and carp tissues have revealed the expression of keratins in both epithelial and mesenchymally derived tissues, as reported previously for zebrafish and trout. We conclude that keratin expression is qualitatively similar among these fishes, with goldfish and carp patterns being more similar to each other than to zebrafish, and the cyprinid fishes being more similar to each other than to the salmonid trout. Because of the detected similarity of keratin expression among the cyprinid fishes, we propose that, for certain experiments, they are interchangeable. Although the zebrafish distinguishes itself as being a developmental and genetic/genomic model organism, we have found that the goldfish, in particular, is a more suitable model for both biochemical and histological studies of the cytoskeleton, especially since goldfish cytoskeletal preparations seem to be more resistant to degradation than those from carp or zebrafish.
Starting Page	245
Ending Page	256
Page Count	12
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	322
Issue Number	2
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-07-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Keratin Intermediate filaments Goldfish (Carassius auratus) Carp (Cyprinus carpio) Zebrafish (Danio rerio) (Teleostei) Molecular Medicine Proteomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

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STUDIES ON THE EFFECT OF CYCLOPOID MEAL INCLUSION IN THE FEEDS OF ORNAMENTAL GOLDFISH (CARASSIUS AURATUS) AND KOI CARP (CYPRINUS CARPIO)

The long-term effect of serotonin on the thermoregulatory behavior in juvenile cyprinidae (Cyprinus carpio and Carassius auratus)

Effects of Pro-Tex on zebrafish (Danio rerio) larvae, adult common carp (Cyprinus carpio) and adult yellowtail kingfish (Seriola lalandi)

Isolation and partial characterization of immunoglobulins of goldfish (Carassius auratus) and carp (Cyprinus carpio)

Calbindin immunoreactivity in the enteric nervous system of larval and adult zebrafish (Danio rerio)

STUDIES ON THE EFFECT OF CAROTENOIDS ON COLORATION, GROWTH AND SURVIVALOF ORNAMENTAL GOLD FISH (CARASSIUS AURATUS), KOI CARP (CYPRINUS CARPIO) AND SWORD TAIL (XIPHOPHOROUS HELLERI)

Comparison of the Exomes of Common Carp (Cyprinus carpio) and Zebrafish (Danio rerio)

Biochemical identification and tissue-specific expression patterns of keratins in the zebrafish Danio rerio

Phylogenetic relationships of silver crusian carp Carassius auratus gibelio, C. auratus cuvieri, crucian carp Carassius carassius, and common carp Cyprinus carpio as inferred from mitochondrial DNA variation

Identification of keratins and analysis of their expression in carp and goldfish: comparison with the zebrafish and trout keratin catalog

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