NDLI: RpoS regulation of gene expression during exponential growth of Escherichia coli K12

Content Provider	Springer Nature Link
Author	Dong, Tao Kirchhof, Mark G. Schellhorn, Herb E.
Copyright Year	2007
Abstract	RpoS is a major regulator of genes required for adaptation to stationary phase in E. coli. However, the exponential phase expression of some genes is affected by rpoS mutation, suggesting RpoS may also have an important physiological role in growing cells. To test this hypothesis, we examined the regulatory role of RpoS in exponential phase using both genomic and biochemical approaches. Microarray expression data revealed that, in the rpoS mutant, the expression of 268 genes was attenuated while the expression of 24 genes was enhanced. Genes responsible for carbon source transport (the mal operon for maltose), protein folding (dnaK and mopAB), and iron acquisition (fepBD, entCBA, fecI, and exbBD) were positively controlled by RpoS. The importance of RpoS-mediated control of iron acquisition was confirmed by cellular metal analysis which revealed that the intracellular iron content of wild type cells was two-fold higher than in rpoS mutant cells. Surprisingly, many previously identified RpoS stationary-phase dependent genes were not controlled by RpoS in exponential phase and several genes were RpoS-regulated only in exponential phase, suggesting the involvement of other regulators. The expression of RpoS-dependent genes osmY, tnaA and malK was controlled by Crl, a transcriptional regulator that modulates RpoS activity. In summary, the identification of a group of exponential phase genes controlled by RpoS reveals a novel aspect of RpoS function.
Starting Page	267
Ending Page	277
Page Count	11
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	279
Issue Number	3
e-ISSN	16174623
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-12-20
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Regulation RpoS Microarray Stress response Exponential phase Animal Genetics and Genomics Plant Genetics & Genomics Microbial Genetics and Genomics Biochemistry Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Control of RpoS in global gene expression of Escherichia coli in minimal media

Characterization and global gene expression of F− phenocopies during Escherichia coli biofilm formation

Microarray analysis of RpoS-mediated gene expression in Escherichia coli K-12

Bacterial luciferase activity and the intracellular redox pool in Escherichia coli

Transcriptomics and adaptive genomics of the asymptomatic bacteriuria Escherichia coli strain 83972

Distinct gene expression signatures during development of distant metastasis

Gene expression in bovine rumen epithelium during weaning identifies molecular regulators of rumen development and growth

Global effect of RpoS on gene expression in pathogenic Escherichia coli O157:H7 strain EDL933

Gene expression profiling during the byssogenesis of zebra mussel (Dreissena polymorpha)

RpoS regulation of gene expression during exponential growth of Escherichia coli K12

Similar Documents

Control of RpoS in global gene expression of Escherichia coli in minimal media

Characterization and global gene expression of F− phenocopies during Escherichia coli biofilm formation

Microarray analysis of RpoS-mediated gene expression in Escherichia coli K-12

Bacterial luciferase activity and the intracellular redox pool in Escherichia coli

Transcriptomics and adaptive genomics of the asymptomatic bacteriuria Escherichia coli strain 83972

Distinct gene expression signatures during development of distant metastasis

Gene expression in bovine rumen epithelium during weaning identifies molecular regulators of rumen development and growth

Global effect of RpoS on gene expression in pathogenic Escherichia coli O157:H7 strain EDL933

Gene expression profiling during the byssogenesis of zebra mussel (Dreissena polymorpha)

RpoS regulation of gene expression during exponential growth of Escherichia coli K12