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| Content Provider | Springer Nature Link |
|---|---|
| Author | Lima, Joel Fernandes Malavazi, Iran Silva Ferreira, Márcia Eliana Savoldi, Marcela Mota, André Oliveira Capellaro, José Luiz Souza Goldman, Maria Helena Goldman, Gustavo Henrique |
| Copyright Year | 2007 |
| Abstract | Nucleotide excision repair (NER) eliminates helix-distorting DNA base lesions. Seven XP-deficient genetic complementation groups (XPA to XPG) have already been identified in mammals, and their corresponding genes have been cloned. Hereditary defects in NER are associated with several diseases, including xeroderma pigmentosum (XP). UV-DDB (XPE) is formed by two associated subunits, DDB1 and DDB2. UV-DDB was identified biochemically as a protein factor that exhibits very strong and specific binding to ultraviolet (UV)-treated DNA. As a preliminary step to characterize the components of the NER in the filamentous fungus Aspergillus nidulans, here we identified a putative DDB1 homologue, DdbA. Deletion and expression analysis indicated that A. nidulans ddbA gene is involved in the DNA damage response, more specifically in the UV light response and 4-nitroquinoline oxide (4-NQO) sensitivity. Furthermore, the ΔddbA strain cannot self-cross and expression analysis showed that ddbA can be induced by oxidative stress and is developmentally regulated in both asexual and sexual processes. The ΔddbA mutation can genetically interact with uvsB ATR, atmAATM, nkuA KU70, H2AX-S129A (a replacement of the conserved serine in the C-terminal of H2AX with alanine), and cshB (a mutation in CSB Cockayne’s syndrome protein involved in the transcription-coupled repair subpathway of NER) mutations. Finally, to determine the DdbA cellular localization, we constructed a GFP::DdbA strain. In the presence and absence of DNA damage, DdbA was mostly detected in the nuclei, indicating that DdbA localizes to nuclei and its cellular localization is not affected by the cellular response to DNA damage induced by 4-NQO and UV light. |
| Starting Page | 239 |
| Ending Page | 253 |
| Page Count | 15 |
| File Format | |
| ISSN | 16174615 |
| Journal | Molecular and General Genetics MGG |
| Volume Number | 279 |
| Issue Number | 3 |
| e-ISSN | 16174623 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2007-11-30 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aspergillus nidulans Nucleotide Excision Repair DDB1 DNA damage binding protein Animal Genetics and Genomics Plant Genetics & Genomics Microbial Genetics and Genomics Biochemistry Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine Molecular Biology |
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