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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ray, Soham Bose, Lotan K. Ray, Joshitha Ngangkham, Umakanta Katara, Jawahar L. Samantaray, Sanghamitra Behera, Lambodar Anumalla, Mahender Singh, Onkar N. Chen, Meingsheng Wing, Rod A. Mohapatra, Trilochan |
| Copyright Year | 2016 |
| Abstract | African wild rice Oryza brachyantha (FF), a distant relative of cultivated rice Oryza sativa (AA), carries genes for pests and disease resistance. Molecular marker assisted alien gene introgression from this wild species to its domesticated counterpart is largely impeded due to the scarce availability of cross-transferable and polymorphic molecular markers that can clearly distinguish these two species. Availability of the whole genome sequence (WGS) of both the species provides a unique opportunity to develop markers, which are cross-transferable. We observed poor cross-transferability (~0.75 %) of O. sativa specific sequence tagged microsatellite (STMS) markers to O. brachyantha. By utilizing the genome sequence information, we developed a set of 45 low cost PCR based co-dominant polymorphic markers (STS and CAPS). These markers were found cross-transferrable (84.78 %) between the two species and could distinguish them from each other and thus allowed tracing alien genome introgression. Finally, we validated a Monosomic Alien Addition Line (MAAL) carrying chromosome 1 of O. brachyantha in O. sativa background using these markers, as a proof of concept. Hence, in this study, we have identified a set molecular marker (comprising of STMS, STS and CAPS) that are capable of detecting alien genome introgression from O. brachyantha to O. sativa. |
| Starting Page | 1783 |
| Ending Page | 1794 |
| Page Count | 12 |
| File Format | |
| ISSN | 16174615 |
| Journal | Molecular and General Genetics MGG |
| Volume Number | 291 |
| Issue Number | 4 |
| e-ISSN | 16174623 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2016-06-14 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Oryza sativa Oryza brachyantha Whole genome sequence (WGS) Cross-transferable co-dominant molecular markers Simple sequence repeats (SSR) Sequence tagged microsatellite (STMS) Sequence tagged sites (STS) Cleaved amplified polymorphic sequence (CAPS) Monosomic alien addition line (MAAL) Relative gene enrichment index (RGEI) Cell Biology Biochemistry Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine Molecular Biology |
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