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An Improved Method of Cleaved Amplified Polymorphic Sequence (CAPS) Markers in Arabidopsis thaliana
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ke, Yuan |
| Copyright Year | 2005 |
| Abstract | The marker enzyme site for each of most CAPS (Cleaved amplified polymorphic sequence) markers could be found in one Arabidopsis ecotype Col (Columbia), only 37.5%～47.5% of analysed enzymes had cut sites in the amplification region of Col genome. These findings indicated that over half of analysed enzymes were not useful in developing most CAPS markers. Based on these findings, an improved CAPS method for development of new markers was established, that is, restriction analysis was conducted at first to find candidate enzymes that have cut sites in Col amplification region, these candidate enzymes were then used to screen CAPS marker enzyme, not using other enzymes unless no polymorphism between ecotypes was detected. Application experiment results showed that the improved method using Col genomic sequence could be used to develop CAPS markers for other two ecotypes Ler (Landsberg erecta) and Cvi (Cape Verde Islands)(of Arabidopis thaliana), even if their genomic sequences were not known; and the cost for over 50% enzymes could be saved. Application of this genome-based economic CAPS (geCAPS) method in other plants such as Oryza sativa and Camellia sinensis were discussed. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.arabidopsis.org/download_files/Protocols/YuanKJ_JAB2005.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
