NDLI: Characterization of the reverse transsulfuration gene mecB of Acremonium chrysogenum, which encodes a functional cystathionine-γ-lyase

Please wait, while we are loading the content...

Multiple cellular processes affected by the absence of the Rpb4 subunit of RNA polymerase II contribute to the deficiency in the stress response of the yeast rpb4 Δ mutant

The mitochondrial inner membrane protein Lpe10p, a homologue of Mrs2p, is essential for magnesium homeostasis and group II intron splicing in yeast

Essential genes in proximal 3L heterochromatin of Drosophila melanogaster

Cycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtii

The chaperonin GroEL and other heat-shock proteins, besides DnaK, participate in ribosome biogenesis in Escherichia coli

Functional analysis of the Bacillus subtilis yshD gene, a mutS paralogue

Cytoplasmic kinase and phosphatase activities can induce PsaF gene expression in the absence of functional plastids: evidence that phosphorylation/dephosphorylation events are involved in interorganellar crosstalk

The mtmVUC genes of the mithramycin gene cluster in Streptomyces argillaceus are involved in the biosynthesis of the sugar moieties

Miscoding and misincorporation of 8-oxo-guanine during leading and lagging strand synthesis in Escherichia coli

A compromised yeast RNA Polymerase II enhances UV sensitivity in the absence of global genome nucleotide excision repair

Isolation and characterization of the fission yeast gene Sprpa12 + reveals that the conserved C-terminal zinc-finger region is dispensable for the function of its product

Characterisation of novel target promoters for the dexamethasone-inducible/tetracycline-repressible regulator TGV using luciferase and isopentenyl transferase as sensitive reporter genes

Isolation, characterization, inheritance and linkage of microsatellite DNA markers in white spruce (Picea glauca) and their usefulness in other spruce species

Functional asymmetry of the two nucleotide binding domains in the ABC transporter Ste6

The Neurospora crassa colonial temperature-sensitive 3 (cot-3) gene encodes protein elongation factor 2

Two RpoH homologs responsible for the expression of heat shock protein genes in Sinorhizobium meliloti

The stringent response, σH-dependent gene expression and sporulation in Bacillus subtilis

DNA photolyase homologs are the major UV resistance factors in the cyanobacterium Synechocystis sp. PCC 6803

Expression of the seqA gene is negatively modulated by the HU protein in Escherichia coli

Molecular and General Genetics MGG : Volume 264, Issue 5, January 2001

Molecular and General Genetics MGG : Volume 264, Issue 4, November 2000

Molecular and General Genetics MGG : Volume 264, Issue 3, October 2000

Molecular and General Genetics MGG : Volume 264, Issue 1-2, September 2000

Molecular and General Genetics MGG : Volume 263

Molecular and General Genetics MGG : Volume 262

Molecular and General Genetics MGG : Volume 261

Molecular and General Genetics MGG : Volume 260

Molecular and General Genetics MGG : Volume 259

Molecular and General Genetics MGG : Volume 258

Molecular and General Genetics MGG : Volume 257

Molecular and General Genetics MGG : Volume 256

Molecular and General Genetics MGG : Volume 255

Molecular and General Genetics MGG : Volume 254

Molecular and General Genetics MGG : Volume 253

Characterization of the reverse transsulfuration gene mecB of Acremonium chrysogenum, which encodes a functional cystathionine-γ-lyase

Content Provider	Springer Nature Link
Author	Marcos, A. T. Kosalková, K. Cardoza, R. E. Fierro, F. Gutiérrez, S. Martín, J. F.
Copyright Year	2000
Abstract	In Acremonium chrysogenum, biosynthesis of cysteine for the formation of cephalosporin has been proposed to occur through the reverse transsulfuration pathway. A gene, named mecB, has been cloned from an A. chrysogenum C10 genomic library in λEMBL3-ble. The cloned DNA fragment encodes a protein of 423 amino acids with a deduced molecular mass of 45 kDa that shows great similarity to cystathionine-γ-lyases from Saccharomyces cerevisiae and other eukaryotic organisms. The protein was shown to be functional because it restores growth on methionine to A. nidulans C47 (mecB10), a mutant that is known to be defective in cystathionine-γ-lyase. The cloned gene did not complement A. nidulans mecA or metG mutants. Enzyme activity assays confirmed that the cloned mecB gene encodes a cystathionine-γ-lyase activity. The mecB gene is present in a single copy in the wild-type A. chrysogenum (Brotzu's strain) and also in the A. chrysogenum strain C10, a high cephalosporin producer. The gene is localized on chromosome VIII (5.3 Mb), as shown by hybridization to A. chrysogenum chromosomes resolved by pulsed-field gel electrophoresis. Transcription of the mecB gene gives rise to a major transcript of 1.5 kb and a minor one of 1.7 kb. The transcript levels were not significantly affected by addition of DL-methionine to the culture, indicating that expression of this gene is not regulated by methionine. The availability of this gene provides a very useful tool for understanding the proposed role of cystathionine-γ-lyase in splitting cystathionine to supply cysteine for cephalosporin biosynthesis.
Starting Page	746
Ending Page	754
Page Count	9
File Format	PDF
ISSN	00268925
Journal	Molecular and General Genetics MGG
Volume Number	264
Issue Number	6
e-ISSN	14321874
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-10-18
Publisher Place	Berlin/Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Genetics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A moderate amplification of the mecB gene encoding cystathionine-γ-lyase stimulates cephalosporin biosynthesis in Acremonium chrysogenum

Targeted Inactivation of the mecB Gene, Encoding Cystathionine-γ-Lyase, Shows that the Reverse Transsulfuration Pathway Is Required for High-Level Cephalosporin Biosynthesis in Acremonium chrysogenum C10 but Not for Methionine Induction of the Cephalosporin Genes

Functional Characterization and Structure-Guided Mutational Analysis of the Transsulfuration Enzyme Cystathionine γ-Lyase from Toxoplasma gondii

Structural basis of the inhibition of cystathionine γ-lyase from Toxoplasma gondii by propargylglycine and cysteine.

Analysis of the sulfur-regulated control of the cystathionine γ-lyase gene of Neurospora crassa

Characterization of the lys2 gene of Acremonium chrysogenum encoding a functional α-aminoadipate activating and reducing enzyme

Aspergillus nidulans genes encoding reverse transsulfuration enzymes belong to homocysteine regulon

Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35

Kinetics and Inhibition of Recombinant Human Cystathionine γ-Lyase TOWARD THE RATIONAL CONTROL OF TRANSSULFURATION

Characterization of the reverse transsulfuration gene mecB of Acremonium chrysogenum, which encodes a functional cystathionine-γ-lyase

Similar Documents

A moderate amplification of the mecB gene encoding cystathionine-γ-lyase stimulates cephalosporin biosynthesis in Acremonium chrysogenum

Targeted Inactivation of the mecB Gene, Encoding Cystathionine-γ-Lyase, Shows that the Reverse Transsulfuration Pathway Is Required for High-Level Cephalosporin Biosynthesis in Acremonium chrysogenum C10 but Not for Methionine Induction of the Cephalosporin Genes

Functional Characterization and Structure-Guided Mutational Analysis of the Transsulfuration Enzyme Cystathionine γ-Lyase from Toxoplasma gondii

Structural basis of the inhibition of cystathionine γ-lyase from Toxoplasma gondii by propargylglycine and cysteine.

Analysis of the sulfur-regulated control of the cystathionine γ-lyase gene of Neurospora crassa

Characterization of the lys2 gene of Acremonium chrysogenum encoding a functional α-aminoadipate activating and reducing enzyme

Aspergillus nidulans genes encoding reverse transsulfuration enzymes belong to homocysteine regulon

Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35

Kinetics and Inhibition of Recombinant Human Cystathionine γ-Lyase TOWARD THE RATIONAL CONTROL OF TRANSSULFURATION

Characterization of the reverse transsulfuration gene mecB of Acremonium chrysogenum, which encodes a functional cystathionine-γ-lyase