NDLI: Isolation and characterization of the fission yeast gene Sprpa12 + reveals that the conserved C-terminal zinc-finger region is dispensable for the function of its product

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Isolation and characterization of the fission yeast gene Sprpa12 + reveals that the conserved C-terminal zinc-finger region is dispensable for the function of its product

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Isolation and characterization of the fission yeast gene Sprpa12 + reveals that the conserved C-terminal zinc-finger region is dispensable for the function of its product

Content Provider	Springer Nature Link
Copyright Year	2000
Abstract	RNA polymerase I of Saccharomyces cerevisiae contains a small subunit, A12.2, encoded by RPA12, that was previously shown to be involved in the assembly and/or stabilization of the largest subunit, A190, of RNA polymerase I. To examine whether an equivalent subunit is present in another eukaryotic RNA polymerase I, we have cloned a Schizosaccahromyces pombe cDNA that is able to complement the rpa12 mutation in S. cerevisiae. The gene, named Sprpa12 +, encodes a polypeptide of 119 amino acids that shows 55% identity to S. cerevisiae A12. 2 over its entire length, including two zinc-finger motifs. Disruption of the chromosomal Sprpa12 + gene shows that it is required for growth at higher temperatures but not at lower temperatures. Expression of Sprpa190 +/nuc1 +, which encodes the largest subunit of the S. pombe RNA polymerase I, from a multicopy plasmid can partially suppress the growth defect of the Sprpa12 disruptant at higher temperatures. These findings suggest that A12.2 subunit is functionally and structurally conserved between S. cerevisiae and S. pombe. Finally, the analysis of mutants suggests that SpRPA12 requires the zinc-finger domain in the N-terminal region but not the one in the C-terminal region for its function.
Starting Page	852
Ending Page	859
Page Count	8
File Format	PDF
ISSN	00268925
Journal	Molecular and General Genetics MGG
Volume Number	264
Issue Number	6
e-ISSN	14321874
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-12-08
Publisher Place	Berlin/Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Genetics

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