NDLI: A non-autonomous insect piggyBac transposable element is mobile in tobacco

Content Provider	Springer Nature Link
Author	Johnson, Eric T. Dowd, Patrick F.
Copyright Year	2014
Abstract	The piggyBac transposable element, originally isolated from a virus in an insect cell line, is a valuable molecular tool for transgenesis and mutagenesis of invertebrates. For heterologous transgenesis in a variety of mammals, transfer of the piggyBac transposable element from an ectopic plasmid only requires expression of piggyBac transposase. To determine if piggyBac could function in dicotyledonous plants, a two-element system was developed in tobacco (Nicotiana tabacum) to test for transposable element excision and insertion. The first transgenic line constitutively expressed piggyBac transposase, while the second transgenic line contained at least two non-autonomous piggyBac transposable elements. Progeny from crosses of the two transgenic lines was analyzed for piggyBac excision and transposition. Several progeny displayed excision events, and all the sequenced excision sites exhibited evidence of the precise excision mechanism characteristic of piggyBac transposase. Two unique transposition insertion events were identified that each included diagnostic duplication of the target site. These data indicate that piggyBac transposase is active in a dicotyledonous plant, although at a low frequency.
Starting Page	895
Ending Page	902
Page Count	8
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	289
Issue Number	5
e-ISSN	16174623
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-05-24
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nicotiana tabacum Transposition PiggyBac Cell Biology Biochemistry Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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