NDLI: Transcriptional regulation of the rat sperm-associated antigen 11e (Spag 11e) gene during endotoxin challenge

Content Provider	Springer Nature Link
Author	Biswas, Barnali Yenugu, Suresh
Copyright Year	2014
Abstract	The lipopolysaccharide (LPS) inducible expression of antimicrobial proteins of the Sperm-Associated Antigen 11 (Spag11) family is dependent on nuclear factor-κB (NF-κB) activation and epigenetic factors. However, the regulatory mechanisms that govern their gene expression during endotoxin challenge are unknown. In this study, we demonstrate that the Spag11e gene upstream sequence contains binding sites for androgen receptor (AR), NF-κB, nuclear factor-1, E-twenty-six and activator protein 2. The role of these transcription factors in inducing Spag11e gene during LPS challenge was analysed by measuring luciferase activity in HEK cells transiently transfected with deletion constructs that lacked one or more of the binding sites. Deletion of AR-binding site resulted in loss of luciferase activity and no further decrease was observed when progressive deletions of the other transcription factor binding sites were made. Mutations in AR or NF-κB binding site resulted in loss of luciferase activity. Electrophoretic gel-mobility shift assays indicated that AR and NF-κB proteins bind to the synthesised radio-labelled oligomers used as probes and the mobility shifted when respective antibodies were added. Results of this study indicate the direct involvement of AR and NF-κB in LPS-induced Spag11e expression, thereby expanding our understanding of antimicrobial gene expression during endotoxin challenge.
Starting Page	837
Ending Page	845
Page Count	9
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	289
Issue Number	5
e-ISSN	16174623
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-04-29
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Androgen receptor Epididymis Sperm-associated antigen 11 Lipopolysaccharide Cell Biology Biochemistry Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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Lipopolysaccharide induces epididymal and testicular antimicrobial gene expression in vitro: insights into the epigenetic regulation of sperm-associated antigen 11e gene

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