NDLI: Molecular characterization of the Aspergillus fumigatus NCS-1 homologue, NcsA

Content Provider	Springer Nature Link
Author	Mota Júnior, André Oliveira Malavazi, Iran Soriani, Frederico Marianetti Heinekamp, Thorsten Jacobsen, Ilse Brakhage, Axel A. Savoldi, Marcela Goldman, Maria Helena S. Silva Ferreira, Márcia Eliana Goldman, Gustavo Henrique
Copyright Year	2008
Abstract	Here, we characterize the Aspergillus fumigatus homologue ncsA Neuronal Calcium Sensor. We showed that ncsA is not an essential gene and ∆ncsA growth was decreased in the presence of EGTA and SDS. Furthermore, the ∆ncsA mutant is more resistant to calcium chloride. NcsA:mRFP localizes to the cytoplasm and its cellular localization is not affected by the cellular response to either calcium chloride or EGTA. The ∆ncsA mutant strain is more sensitive to voriconazole, itraconazole, and amphotericin. Polar growth in the ΔncsA mutant was also considerably more affected by lovastatin than in the wild type strain. The Spitzenkörper can be visualized in both strains and although the vacuolar system does not seem to be very different, there is an increase in the staining intensity on the germling surface of the ∆ncsA strain. NcsA promotes pmcA and pmcB expression and therefore there is a reduced expression of these ion pumps in the ΔncsA mutant background, and also of other genes involved in the response to calcium in A. fumigatus. The ncsA inactivation mutation is not causing loss of virulence in a low dose murine infection when compared to the corresponding wild type strain.
Starting Page	483
Ending Page	495
Page Count	13
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	280
Issue Number	6
e-ISSN	16174623
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-10-02
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neuronal Calcium Sensor Aspergillus fumigatus Calcium Animal Genetics and Genomics Microbial Genetics and Genomics Cell Biology Plant Genetics & Genomics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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