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| Content Provider | Springer Nature Link |
|---|---|
| Author | Tierney, J. Mulcahy, G. |
| Copyright Year | 2003 |
| Abstract | Attempts to propagate Eimeria tenella in cell culture over the years have met with limited success. The host cell type is an important parameter in such cultures. This study assessed the ability of different host cell lines to support E. tenella infection in vitro. The initial development in cell lines and the effects of incubation at 37°C and 41°C on the host-parasite relationship was investigated. Eleven cell lines were seeded into 96 well plates and incubated at 37°C in 5% CO2 to reach confluency. Sporozoites of E. tenella were inoculated into wells and allowed to invade at 37°C and 41°C. Intracellular parasite development was quantified using 3H-uracil incorporation. All cell lines facilitated parasite invasion and development. The MDBK cell line supported the highest degree of E. tenella development. A time-course study was undertaken to look at this host cell-parasite relationship during infection. |
| Starting Page | 301 |
| Ending Page | 304 |
| Page Count | 4 |
| File Format | |
| ISSN | 09320113 |
| Journal | Parasitology Research |
| Volume Number | 90 |
| Issue Number | 4 |
| e-ISSN | 14321955 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2003-04-01 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Veterinary Parasitology Insect Science |
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