NDLI: Intracellular localization of viral RNA in Eimeria necatrix of the domestic fowl

Content Provider	Springer Nature Link
Author	Lee, Susan Fernando, M. A.
Copyright Year	1998
Abstract	The intracellular localization of viral RNA in three different stages of Eimeria necatrix, namely, the first- and second-generation meronts and the macrogamonts, were examined at the light microscopy level by in situ hybridization. Digoxigenin-labeled riboprobes generated from partial cDNA clones from 5.6-kb and 4.5-kb dsRNA (pzenv1 and pzenv2) were used in this study. Viral RNA was found to be confined to the cytoplasm of the eimerian host; no viral RNA was detected in chicken tissue. The intense hybridization signal observed in the cytoplasm of the immature meronts with the SP6 riboprobe of pzenv1 or the T7 riboprobe of pzenv2 was probably due to their hybridization to positive strands that had been extruded from the virus during transcription. In mature meronts the intensity of the signal is lower, signifying a decrease in transcription activity. Viral replicase activity may thus be synchronized with the growth phase of the eimerian host.
Starting Page	601
Ending Page	606
Page Count	6
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	84
Issue Number	8
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-07-27
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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Intracellular localization of viral RNA in Eimeria necatrix of the domestic fowl