NDLI: Development of an enzyme-linked immunosorbent assay for detection of antibodies to Babesia bigemina in cattle

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Development of an enzyme-linked immunosorbent assay for detection of antibodies to Babesia bigemina in cattle

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Development of an enzyme-linked immunosorbent assay for detection of antibodies to Babesia bigemina in cattle

Content Provider	Springer Nature Link
Author	Molloy, J. B. Bowles, P. M. Jeston, P. J. Bruyeres, A. G. Bowden, J. M. Bock, R. E. Jorgensen, W. K. Blight, G. W. Dalgliesh, R. J.
Copyright Year	1998
Abstract	Monoclonal antibodies, directed against a 58-kDa Babesia bigemina merozoite antigen that reacted strongly with immune sera from experimentally and naturally infected cattle in Western blots, were used to develop a competitive-inhibition enzyme-linked immunosorbent assay (ELISA). As based on the testing of 70 antibody-positive sera from experimentally infected cattle and 166 antibody-negative sera collected in non-endemic areas of Australia, the sensitivity and specificity of the ELISA were 95.7% and 97.0%, respectively. In sequential sera collected from six calves during the course of experimental B. bigemina infections the ELISA detected seroconversion at about 10 days post-inoculation. The specificity of the ELISA was not affected by the presence of antibodies to B. bovis, Anaplasma marginale or Theileria buffeli. In 42 sera from cattle experimentally infected with B. bovis but negative for B. bigemina the specificity of the ELISA was 95.2%. The use of a competitive-inhibition ELISA format detecting only antibody directed against a single epitope on the 58-kDa antigen appears to have overcome many of the specificity problems that have plagued serological tests for B. bigemina in the past. The test should be useful for epidemiology studies, particularly in areas where B. bovis and B. bigemina have overlapping distributions.
Starting Page	651
Ending Page	656
Page Count	6
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	84
Issue Number	8
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-07-27
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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