NDLI: Mapping the genome of Eimeria tenella : use of a yeast artificial chromosome library as the basis for a physical map

Content Provider	Springer Nature Link
Author	Hoogendoorn, B. Toye, A. A. Bumstead, N. Shirley, M. W.
Copyright Year	1997
Abstract	Efforts to derive a physical map of the genome of Eimeria tenella are being made to complement genetic mapping and to assist in the production of an integrated map. A large insert library of DNA from the Houghton (H) reference strain of E. tenella has been constructed in yeast artificial chromosomes (YACs). The library contains a tenfold E. tenella genome equivalent consisting of 3177 clones arrayed in 96-well microtitre plates and gridded on nylon membranes at 1728 clones/86 cm2. Size fractionation of a random sample of 185 YACs revealed an average insert size of approximately 170 kb. Hybridisation of four E. tenella single-copy probes to the gridded colony filters produced around four or more positive clones. A probe representing the whole of the 1.2-Mb chromosome 2, excised from a preparative gel following pulsed-field gel electrophoresis, revealed between 80 and 100 positive clones.
Starting Page	13
Ending Page	16
Page Count	4
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	84
Issue Number	1
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	1997-11-17
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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Mapping the genome of Eimeria tenella : use of a yeast artificial chromosome library as the basis for a physical map