NDLI: Preliminary characterization of an adult worm “vomit” preparation of Schistosoma mansoni and its potential use as antigen for diagnosis

Content Provider	Springer Nature Link
Author	Planchart, Sandra Incani, Renzo Ni Cesari, Italo Mario
Copyright Year	2007
Abstract	Schistosoma mansoni is a parasitic trematode of the portal–mesenteric veins with a closed-end intestine. Adult worms regurgitate their intestinal content after digestion, together with constituents of the lining gut. Some of these molecules circulate in the blood and are antigenic. We obtain a “vomit” preparation and preliminary evaluate its biochemical composition and antigenic capacity. The “vomit” preparation was obtained after changes in temperature and solutions of incubation of adult worms between 4 and 37°C. Supernatant was assayed for protein, carbohydrate concentration and enzymatic activities associated to the intestine and to the worm tegument. The antigenicity of the product was evaluated using Western blot (WB) analysis against sera of experimentally infected mice, before and after drug cure, sera from people infected with S. mansoni and from individuals infected with other parasitoses. More carbohydrate than protein was detected in the preparations. Cysteine proteinase (CP), N-acetyl-β-d-glucosaminidase and alkaline phosphatase activities were detected. The latter enzyme activity is a marker of the tegument, suggesting that in spite of careful conditions used to avoid the presence of tegumental material, manipulation of the worms always resulted in the release of tegumental molecules. Cationic exchange chromatography was useful to separate various components of this “vomit” preparation, particularly enzymes responsible for CP activity. Two highly immunogenic and specific duplets were observed in the WB analysis, 31/32- and 38/40-kDa components, the former probably referring to the intestinal CPs Sm31/Sm32. None of the two duplets disappeared after successful chemotherapy during the time of evaluation in mice or humans.
Starting Page	301
Ending Page	309
Page Count	9
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	101
Issue Number	2
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-02-20
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunology Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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