NDLI: Isoforms of Hsp70-binding human LDL in adult Schistosoma mansoni worms

Content Provider	Springer Nature Link
Author	Pereira, Adriana S. A. Cavalcanti, Marília G. S. Zingali, Russolina B. Lima Filho, José L. Chaves, Maria E. C.
Copyright Year	2015
Abstract	Schistosoma mansoni is one of the most common parasites infecting humans. They are well adapted to the host, and this parasite’s longevity is a consequence of effective escape from the host immune system. In the blood circulation, lipoproteins not only help to conceal the worm from attack by host antibodies but also act as a source of lipids for S. mansoni. Previous SEM studies showed that the low-density lipoprotein (LDL) particles present on the surface of adult S. mansoni worms decreased in size when the incubation time increased. In this study, immunocytochemical and proteomic analyses were used to locate and identify S. mansoni binding proteins to human plasma LDL. Ultrathin sections of adult worms were cut transversely from the anterior, medial and posterior regions of the parasite. Immunocytochemical experiments revealed particles of gold in the tegument, muscle region and spine in male worms and around vitelline cells in females. Immunoblotting and 2D-electrophoresis using incubations with human serum, anti-LDL antibodies and anti-chicken IgG peroxidase conjugate were performed to identify LDL-binding proteins in S. mansoni. Analysis of the binding proteins using LC-MS identified two isoforms of the Hsp70 chaperone in S. mansoni. Hsp70 is involved in the interaction with apoB in the cytoplasm and its transport to the endoplasmic reticulum. However, further studies are needed to clarify the functional role of Hsp70 in S. mansoni, mainly related to the interaction with human LDL.
Starting Page	1145
Ending Page	1152
Page Count	8
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	114
Issue Number	3
e-ISSN	14321955
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-01-10
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Schistosoma Schistosoma mansoni Lipoproteins LDL Hsp70 Medical Microbiology Microbiology Immunology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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