NDLI: Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation

Content Provider	Springer Nature Link
Author	Pandolfi, Matías Lo stro, Fabiana L. Shimizu, Akio Pozzi, Andrea G. Meijide, Fernando J. Vazquez, Graciela Rey Maggese, M. Cristina
Copyright Year	2006
Abstract	Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) expressing cells were detected in pituitary, brain and ovary of the Perciform cichlid fish Cichlasoma dimerus. This detection was carried out by immunohistochemistry (IHC) and Western blot techniques using antisera of the Cyprinodontiform Fundulus heteroclitus raised against the conservative region of the teleost βFSH and the βLH subunits. The estimated molecular weights were 24 kDa for LH and 19 and 15 kDa for FSH. In the adult pituitary, both cell types were distributed along mid and ventral zones of the proximal pars distalis (PPD, mid-immunoreactive cells), and along the ventral and dorsal external border of the pars intermedia (PI, high-immunoreactive cells). Double IHC showed that FSH and LH are mainly expressed in different pituitary cells. FSH cells were detected in the pituitary around day 21 after hatching (ah) (prior to sex differentiation), while LH cells were detected by day 60 ah (during the sexual differentiation period). A correlation between gonadal sex differentiation and FSH was demonstrated in a 15 days organ culture system. FSH and LH neurons were localized in the nucleus lateralis tuberis and their fibers project through the ventral hypothalamus, preoptic area and neurohypophysis. FSH neurons differentiated on day 21 ah, while LH neurons appeared on day 15 ah. In the ovary, the immunoreactivity for both FSH and LH was restricted to the cytoplasm of previtellogenic and early vitellogenic oocytes.
Starting Page	355
Ending Page	365
Page Count	11
File Format	PDF
ISSN	03402061
Journal	Anatomy and Embryology
Volume Number	211
Issue Number	5
e-ISSN	14320568
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-05-05
Publisher Place	Berlin/Heidelberg
Access Restriction	Subscribed
Subject Keyword	Teleostei Cichlidae Gonadotropins Sexual differentiation Ontogeny Neurology Cell Biology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Anatomy Cell Biology Developmental Biology Embryology

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