NDLI: The Drosophila jing gene is a downstream target in the Trachealess/Tango tracheal pathway

Content Provider	Springer Nature Link
Author	Morozova, Tatiana Hackett, Joanne Sedaghat, Yalda Sonnenfeld, Margaret
Copyright Year	2010
Abstract	Primary branching in the Drosophila trachea is regulated by the Trachealess (Trh) and Tango (Tgo) basic helix-loop-helix-PAS (bHLH-PAS) heterodimers, the POU protein Drifter (Dfr)/Ventral Veinless (Vvl), and the Pointed (Pnt) ETS transcription factor. The jing gene encodes a zinc finger protein also required for tracheal development. Three Trh/Tgo DNA-binding sites, known as CNS midline elements, in 1.5 kb of jing 5′ cis-regulatory sequence (jing1.5) previously suggested a downstream role for jing in the pathway. Here, we show that jing is a direct downstream target of Trh/Tgo and that Vvl and Pnt are also involved in jing tracheal activation. In vivo lacZ enhancer detection assays were used to identify cis-regulatory elements mediating embryonic expression patterns of jing. A 2.8-kb jing enhancer (jing2.8) drove lacZ expression in all tracheal cell lineages, the CNS midline and Engrailed-positive segmental stripes, mimicking endogenous jing expression. A 1.3-kb element within jing2.8 drove expression that was restricted to Engrailed-positive CNS midline cells and segmental ectodermal stripes. Surprisingly, jing1.5-lacZ expression was restricted to tracheal fusion cells despite the presence of consensus DNA-binding sites for bHLH-PAS, ETS, and POU domain transcription factors. Given the absence of Trh/Tgo DNA-binding sites in the jing1.3 enhancer, these results are consistent with previous observations suggesting a combinatorial basis to Trh-/Tgo-mediated transcriptional regulation in the trachea.
Starting Page	191
Ending Page	206
Page Count	16
File Format	PDF
ISSN	0949944X
Journal	Development Genes and Evolution
Volume Number	220
Issue Number	7-8
e-ISSN	1432041X
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-11-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Drosophila melanogaster Tracheal branching Trachealess/Tango Transcriptional regulation jing bHLH-PAS dfr/vvl POU transcription factor Tyrosine kinase signaling Animal Genetics and Genomics Neurosciences Biochemistry Cell Biology Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Developmental Biology

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