NDLI: Structurally related Arabidopsis ANGUSTIFOLIA is functionally distinct from the transcriptional corepressor CtBP

Content Provider	Springer Nature Link
Author	Stern, Mark D. Aihara, Hitoshi Cho, Kiu Hyung Kim, Gyung Tae Horiguchi, Gorou Roccaro, Giorgio A. Guevara, Elizabeth Sun, Huan Huan Negeri, Dereje Tsukaya, Hirokazu Nibu, Yutaka
Copyright Year	2007
Abstract	ANGUSTIFOLIA (AN) controls leaf morphology in the plant Arabidopsis thaliana. Previous studies on sequence similarity demonstrated that the closest proteins to AN are members of animal C-terminal-binding proteins (CtBPs) found in nematodes, arthropods, and vertebrates. Drosophila CtBP (dCtBP) functions as a transcriptional corepressor for deoxyribonucleic acid (DNA)-binding repressors containing the short amino acid motif, PXDLS, to regulate tissue specification and segmentation during early embryogenesis. It has previously been shown that AN was thought to repress transcription similar to the function of CtBPs; however, AN lacks some of the structural features that are conserved in animal CtBPs. In this paper, we examined whether AN is functionally related to dCtBP. Firstly, we re-examined sequence similarity among AN and various CtBPs from several representative species in the plant and animal kingdoms. Secondly, yeast two-hybrid assays demonstrated that AN failed to interact with an authentic CtBP-interacting factor, adenovirus E1A oncoprotein bearing the PXDLS motif. Thirdly, AN tethered to DNA was unable to repress the expression of reporter genes in transgenic Drosophila embryos. Fourthly, overexpression assays suggested that dCtBP and AN function differently in Drosophila tissues. Finally, AN failed to rescue the zygotic lethality caused by dCtBP loss-of-function. These data, taken together, suggest that AN is functionally distinct from dCtBP. Likely, ancestral CtBPs acquired corepressor function (capability of both repression and binding to repressors containing the PXDLS motif) after the animal–plant divergence but before the protostome–deuterostome split. We therefore propose to categorize AN as a subfamily member within the CtBP/BARS/RIBEYE/AN superfamily.
Starting Page	759
Ending Page	769
Page Count	11
File Format	PDF
ISSN	0949944X
Journal	Development Genes and Evolution
Volume Number	217
Issue Number	11-12
e-ISSN	1432041X
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-10-31
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	ANGUSTIFOLIA Arabidopsis thaliana Corepressor CtBP Drosophila melanogaster Animal Genetics and Genomics Biochemistry Cell Biology Neurosciences Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Developmental Biology

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