NDLI: RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content

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RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content

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RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content

Content Provider	Springer Nature Link
Author	Nunes, Aline C. S. Vianna, Giovanni R. Cuneo, Florencia Amaya Farfán, Jaime Capdeville, Guy Rech, Elíbio L. Aragão, Francisco J. L.
Copyright Year	2005
Abstract	Inositol plays a role in membrane trafficking and signaling in addition to regulating cellular metabolism and controlling growth. In plants, the myo-inositol-1-phosphate is synthesized from glucose 6-phosphate in a reaction catalyzed by the enzyme myo-inositol-1-phosphate synthase (EC 5.5.1.4). Inositol can be converted into phytic acid (phytate), the most abundant form of phosphate in seeds. The path to phytate has been suggested to proceed via the sequential phosphorylation of inositol phosphates, and/or in part via phosphatidylinositol phosphate. Soybean [Glycine max (L.) Merrill] lines were produced using interfering RNA (RNAi) construct in order to silence the myo-inositol-1-phosphate (GmMIPS1) gene. We have observed an absence of seed development in lines in which the presence of GmMIPS1 transcripts was not detected. In addition, a drastic reduction of phytate (InsP6) content was achieved in transgenic lines (up to 94.5%). Our results demonstrated an important correlation between GmMIPS1 gene expression and seed development.
Starting Page	125
Ending Page	132
Page Count	8
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	224
Issue Number	1
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-01-04
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Glycin Inositol myo-Inositol 1-phosphate synthase Phytic acid Phytin globoid RNA-interference Seed development
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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