NDLI: Changes in chloroplast DNA during development in tobacco, Medicago truncatula, pea, and maize

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Changes in chloroplast DNA during development in tobacco, Medicago truncatula, pea, and maize

Content Provider	Springer Nature Link
Author	Shaver, Jeffrey M. Oldenburg, Delene J. Bendich, Arld J.
Copyright Year	2005
Abstract	We examined the DNA from chloroplasts obtained from young and fully expanded leaves of tobacco (Nicotiana tabacum L.), Medicago truncatula, pea (Pisum sativum L.), and maize (Zea mays L.). The changes in plastid DNA content and structure were monitored by four independent methods: 4′,6-diamidino-2-phenylindole (DAPI) staining with intact chloroplasts, in situ DAPI staining of cytological sections, ethidium bromide staining at the single-molecule level after exhaustive deproteinization of lysed chloroplasts, and pulsed-field gel electrophoresis. During leaf development, we found a decline of chloroplast DNA (cpDNA) in all four plants. For tobacco, for which plants can readily be regenerated from somatic cells, cpDNA persisted longer than in the other three plants. We also found a striking progression from complex multigenomic DNA molecules to simple subgenomic molecules during plastid development. Although the decrease in molecular size and complexity paralleled the decrease in DNA content per plastid, 6% of the chloroplasts in a fully expanded tobacco leaf still contained DNA in complex branched structure, whereas no such complex structures were found in mature leaves for the hard-to-regenerate maize.
Starting Page	72
Ending Page	82
Page Count	11
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	224
Issue Number	1
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-12-15
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chloroplast DNA DAPI Development Fluorescence microscopy Pulsed-field gel electrophoresis Regeneration
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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