NDLI: High expression level of a foot and mouth disease virus epitope in tobacco transplastomic plants

Content Provider	Springer Nature Link
Author	Lentz, Ezequiel Matías Segretin, María Eugenia Morgenfeld, Mauro Miguel Wirth, Sonia Alejandra Dus Santos, María José Mozgovoj, Marina Valeria Wigdorovitz, Andrés Bravo Almonacid, Fernando Félix
Copyright Year	2009
Abstract	Chloroplast transformation has an extraordinary potential for antigen production in plants because of the capacity to accumulate high levels of recombinant proteins and increased biosafety due to maternal plastid inheritance in most crops. In this article, we evaluate tobacco chloroplasts transformation for the production of a highly immunogenic epitope containing amino acid residues 135–160 of the structural protein VP1 of the foot and mouth disease virus (FMDV). To increase the accumulation levels, the peptide was expressed as a fusion protein with the β-glucuronidase reporter gene (uidA). The recombinant protein represented the 51% of the total soluble proteins in mature leaves, a level higher than those of the Rubisco large subunit, the most abundant protein in the leaf of a wild-type plant. Despite this high accumulation of heterologous protein, the transplastomic plants and wild-type tobacco were phenotypically indistinguishable. The FMDV epitope expressed in transplastomic plants was immunogenic in mice. These results show that transplastomic tobacco express efficiently the recombinant protein, and we conclude that this technology allows the production of large quantities of immunogenic proteins.
Starting Page	387
Ending Page	395
Page Count	9
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	231
Issue Number	2
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-11-22
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antigen Foot and mouth disease virus Molecular farming Plastid transformation Tobacco Ecology Forestry Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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