NDLI: Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata

Content Provider	Springer Nature Link
Author	Xu, Weirong Yu, Yihe Ding, Jiahua Hua, Zhanyong Wang, Yuejin
Copyright Year	2009
Abstract	Stilbene synthase is a plant-specific polyketide synthase, and plays important roles in diverse metabolic processes. The genomic stilbene synthase gene was cloned from accession “Baihe-35-1” of Chinese wild Vitis pseudoreticulata, and a stilbene synthase of V. pseudoreticulata (VpSTS) transcripts expressed in the grape–powdery mildew interaction were determined by semi-quantitative RT-PCR. To monitor VpSTS expression in plant, the promoter region flanking the 5′ VpSTS coding region was isolated from the genomic DNA of Chinese wild V. pseudoreticulata accession Baihe-35-1. Alignment of the VpSTS promoter sequence showed a 56.4% identity to Vitis vinifera. To identify the upstream region of the VpSTS gene required for promoter activity, a series of VpSTS promoter deletion derivatives was constructed. Each deletion construct was analyzed by Agrobacterium-mediated transient transformation in grapevine and tobacco leaves after infection by Uncinula necator and Alternaria alternata. In transiently transformed grapevine leaves, GUS activity was also determined after treatment with salicylic acid (SA) and 4°C cold. Analysis of a series of 5′ deletions of the VpSTS promoter in grapevine leaves indicated that the proximal 162 bp from the transcription initiation site was proved to be necessary for establishing both the constitutive and induced pattern of expression.
Starting Page	475
Ending Page	487
Page Count	13
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	231
Issue Number	2
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-11-24
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chinese wild Vitis Pathogen- and stress-inducible gene Promoter analysis Stilbene synthase Uncinula Ecology Forestry Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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