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| Content Provider | Springer Nature Link |
|---|---|
| Author | Gilson, Paul R. Vergara, Claudia E. Kjer Nielsen, Lars Teasdale, Rohan D. Bacic, Antony Gleeson, Paul A. |
| Copyright Year | 2004 |
| Abstract | A family of Golgi-localised molecules was recently described in animals and fungi possessing extensive coiled regions and a short (~40 residues) conserved C-terminal domain, called the GRIP domain, which is responsible for their location to this organelle. Using the model plant Arabidopsis thaliana, we identified a gene (AtGRIP) encoding a putative GRIP protein. We demonstrated that the C-terminal domain from AtGRIP functions as a Golgi-targeting sequence in plant cells. Localisation studies in living cells expressing the AtGRIP fused to a DsRed2 fluorescent probe, showed extensive co-location with the Golgi marker α-mannosidase I in transformed tobacco protoplasts. GRIP-like sequences were also found in genomic databases of rice, maize, wheat and alfalfa, suggesting that this domain may be a useful Golgi marker for immunolocalisation studies. Despite low sequence identity amongst GRIP domains, the plant GRIP sequence was able to target to the Golgi of mammalian cells. Taken together, these data indicate that GRIP domain proteins might be implicated in a targeting mechanism that is conserved amongst eukaryotes. |
| Starting Page | 1050 |
| Ending Page | 1056 |
| Page Count | 7 |
| File Format | |
| ISSN | 00320935 |
| Journal | Planta |
| Volume Number | 219 |
| Issue Number | 6 |
| e-ISSN | 14322048 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2004-06-22 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Arabidopsis Golgi apparatus Golgin GRIP domain |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Plant Science |
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