NDLI: Substituent-specific antibody against glucuronoxylan reveals close association of glucuronic acid and acetyl substituents and distinct labeling patterns in tree species

Content Provider	Springer Nature Link
Author	Koutaniemi, Sanna Guillon, Fabienne Tranquet, Olivier Bouchet, Brigitte Tuomainen, Päivi Virkki, Liisa Petersen, Henriette L. Willats, William G. T. Saulnier, Luc Tenkanen, Maija
Copyright Year	2012
Abstract	Immunolabeling can be used to locate plant cell wall carbohydrates or other components to specific cell types or to specific regions of the wall. Some antibodies against xylans exist; however, many partly react with the xylan backbone and thus provide limited information on the type of substituents present in various xylans. We have produced a monoclonal antibody which specifically recognizes glucopyranosyl uronic acid (GlcA), or its 4-O-methyl ether (meGlcA), substituents in xylan and has no cross-reactivity with linear or arabinofuranosyl-substituted xylans. The UX1 antibody binds most strongly to (me)GlcA substitutions at the non-reducing ends of xylan chains, but has a low cross-reactivity with internal substitutions as well, at least on oligosaccharides. The antibody labeled plant cell walls from both mono- and dicotyledons, but in most tissues an alkaline pretreatment was needed for antibody binding. The treatment removed acetyl groups from xylan, indicating that the vicinity of glucuronic acid substituents is also acetylated. The novel labeling patterns observed in the xylem of tree species suggested that differences within the cell wall exist both in acetylation degree and in glucuronic acid content.
Starting Page	739
Ending Page	751
Page Count	13
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	236
Issue Number	2
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-04-24
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	4-O-methylglucuronic acid Antibody specificity Immunolocalization NMR Xylan acetylation Xylooligosaccharides Forestry Ecology Plant Sciences Agriculture
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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