NDLI: Immunolocalization and structural variations of xylan in differentiating earlywood tracheid cell walls of Cryptomeria japonica

Content Provider	Springer Nature Link
Author	Kim, Jong Sik Awa, Tatsuya Yoshinaga, Arata Takabe, Keiji
Copyright Year	2010
Abstract	We investigated the spatial and temporal distribution of xylans in the cell walls of differentiating earlywood tracheids of Cryptomeria japonica using two different types of monoclonal antibodies (LM10 and LM11) combined with immunomicroscopy. Xylans were first deposited in the corner of the S1 layer in the early stages of S1 formation in tracheids. Cell corner middle lamella also showed strong xylan labeling from the early stage of cell wall formation. During secondary cell wall formation, the innermost layer and the boundary between the S1 and S2 layers (S1/S2 region) showed weaker labeling than other parts of the cell wall. However, mature tracheids had an almost uniform distribution of xylans throughout the entire cell wall. Xylan localization labeled with LM10 antibody was stronger in the outer S2 layer than in the inner layer, whereas xylans labeled with LM11 antibody were almost uniformly distributed in the S2 layer. In addition, the LM10 antibody showed almost no xylan labeling in the S1/S2 region, whereas the LM11 antibody revealed strong xylan labeling in the S1/S2 region. These findings suggest that structurally different types of xylans may be deposited in the tracheid cell wall depending on the developmental stage of, or location in, the cell wall. Our study also indicates that deposition of xylans in the early stages of tracheid cell wall formation may be spatially consistent with the early stage of lignin deposition in the tracheid cell wall.
Starting Page	817
Ending Page	824
Page Count	8
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	232
Issue Number	4
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-07-14
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cryptomeria japonica Immunolocalization LM10 antibody LM11 antibody Xylan Ecology Forestry Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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