NDLI: Enhanced Ca2+-induced Ca2+ release from intracellular stores contributes to catecholamine hypersecretion in adrenal chromaffin cells from spontaneously hypertensive rats

Content Provider	Springer Nature Link
Author	Segura Chama, Pedro López Bistrain, Patricia Pérez Armendáriz, Elia Martha Jiménez Pérez, Nicolás Millán Aldaco, Diana Hernández Cruz, Arturo
Copyright Year	2015
Abstract	Adrenal chromaffin cells (CCs) from spontaneously hypertensive rats (SHRs) secrete more catecholamine (CA) upon stimulation than CCs from normotensive Wistar Kyoto rats (WKY). Unitary CA exocytosis events, both spontaneous and stimulated, were amperometrically recorded from cultured WKY and SHR CCs. Both strains display spontaneous amperometric spikes but SHR CCs produce more spikes and of higher mean amplitude. After a brief stimulation with high K+ or caffeine which produces voltage-gated Ca2+ influx or intracellular Ca2+ release, respectively, more spikes and of greater mean amplitude and unitary charge were recorded in SHR CCs. Consequently, peak cumulative charge was ~2-fold higher in SHR CCs. Ryanodine (10 μM), a specific blocker of the ryanodine receptors reduced depolarization-induced peak cumulative charge by ~10 % in WKY and ~77 % in SHR CCs, suggesting, a larger contribution of Ca2+-induced Ca2+ release to CA exocytosis in SHR CCs. Accordingly, Ca2+ imaging showed larger [Ca2+]i signals induced both by depolarization and caffeine in SHR CCs. Distribution amplitude histograms showed that small amperometric spikes (0–50 pA) are more frequent in WKY than in SHR CCs. Conversely, medium (50–190 pA) and large (190–290 pA) spikes are more numerous in SHR than in WKY CCs. This study reveals that the enhanced CA secretion in SHR CCs results from a combination of (1) larger depolarization-induced Ca2+ transients, due to a greater Ca2+-induced intracellular Ca2+ release, (2) more exocytosis events per time unit, and (3) a greater proportion of medium and large amperometric spikes probably due to a higher mean CA content per granule. Enhanced CA release by excessive amplification by Ca2+ induced Ca2+ release and larger granule catecholamine content contributes to the increased CA plasma levels and vasomotor tone in SHRs.
Starting Page	2307
Ending Page	2323
Page Count	17
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	467
Issue Number	11
e-ISSN	14322013
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-03-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Catecholamine release Chromaffin cell Endoplasmic reticulum Calcium-induced calcium release Spontaneously hypertensive rat Amperometry Human Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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