NDLI: Microdialysis of insulin-like growth factor-I in human muscle

Content Provider	Springer Nature Link
Author	Desvigne, N. Barthélémy, J. C. Frère, D. Gay Montchamp, J. P. Costes, F.
Copyright Year	2005
Abstract	The determination of the insulin like growth factor I (IGF-I) concentration in the interstitial milieu is of outstanding importance to explore its autocrine/paracrine function. We previously reported a method to calibrate microdialysis probes for lactate and glucose (slope method). In the present study, we investigated the ability of our method to determine the concentration of larger molecules, such as IGF-I. We observed in vitro a close linear relationship (r=0.86, P<0.0005) between the recoveries of lactate (RecLac) and of IGF-I (RecIGF-I), giving access to the measurement of IGF-I with the same accuracy as the one previously found for lactate and glucose. In seven human volunteers, we calibrated each probe with the slope method: we first determined in vivo for every probe the specific RecLac/loss ethanol relationship and thereafter, using that relationship, we deduced RecLac from the loss ethanol value measured in every dialysate. This allowed calculation of RecIGF-I from the calculated RecLac value and the in vitro RecLac/RecIGF-I relationship, and finally free IGF-I concentration in muscle interstitial fluid. The mean free IGF-I interstitial concentration was 6.8±3.2 ng/ml while the mean plasma concentration was 0.4±0.2 ng/ml. This large gradient from interstitium to plasma for free IGF-I could be related to the local action of this growth factor.
Starting Page	216
Ending Page	219
Page Count	4
File Format	PDF
ISSN	14396319
Journal	European Journal of Applied Physiology
Volume Number	94
Issue Number	1
e-ISSN	14396327
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-01-29
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microdialysis Muscle Growth factor
Content Type	Text
Resource Type	Article
Subject	Orthopedics and Sports Medicine Physiology (medical) Public Health, Environmental and Occupational Health Sports Science

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