NDLI: Exercise-induced changes in circulating levels of transforming growth factor-β-1 in humans: methodological considerations

Content Provider	Springer Nature Link
Author	Heinemeier, Katja Langberg, Henning Kjaer, Michael
Copyright Year	2003
Abstract	Mechanical loading of cells induces the expression of transforming growth factor-β-1, and acute exercise, which involves mechanical loading of several tissues, could thus increase its circulating level in humans. However, no consensus exists regarding the plasma concentration of this cytokine in resting subjects (reported values range from 500 to 18,300 pg ml−1) and also the extent of intra-individual variation is unknown. As a basis for detecting exercise-induced changes in transforming growth factor-β-1, we measured its concentration, by enzyme-linked immunosorbent assay, in plasma from eight healthy resting subjects. Plasma was sampled from each subject on five successive days according to a procedure designed to minimize activation of platelets, as platelet α-granules contain large amounts of transforming growth factor-β-1. The mean plasma level was relatively low [1155 (30) pg ml−1, mean (SE)], and did not differ between days, indicating that platelet activation was minimal. Several alterations in the blood sampling procedure did not affect results, while a 40% increase was seen when blood was not cooled appropriately prior to centrifugation. A moderate intra-individual variation (average CV=9.8%) indicated a stable plasma level at rest. In response to exercise (1 h of treadmill running) the plasma concentration of transforming growth factor-β-1 increased from 992 (49) pg ml−1 (at rest) to 1301 (39) pg ml−1 (post exercise) (P<0.05) (n=6). In conclusion, the resting plasma level of transforming growth factor-β-1 was stable over time when blood samples were treated appropriately. Exercise increased the plasma concentration, perhaps indicating a release from mechanically loaded tissues.
Starting Page	171
Ending Page	177
Page Count	7
File Format	PDF
ISSN	14396319
Journal	European Journal of Applied Physiology
Volume Number	90
Issue Number	1
e-ISSN	14396327
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-07-09
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Exercise Plasma Platelets TGF-β-1
Content Type	Text
Resource Type	Article
Subject	Orthopedics and Sports Medicine Physiology (medical) Public Health, Environmental and Occupational Health Sports Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Exercise-induced changes in circulating levels of transforming growth factor-β-1 in humans: methodological considerations

Circulating transforming growth factor β1 (TGFβ1) is elevated by extensive exercise

Exercise-induced shear stress is associated with changes in plasma von Willebrand factor in older humans

Exercise-induced changes in blood levels of α-tocopherol

HGF suppresses the production of collagen type III and α-SMA induced by TGF-β1 in healing fibroblasts

HGF inhibits TGF-β1-induced myofibroblast differentiation and ECM deposition via MMP-2 in Achilles tendon in rat

Exercise-induced shear stress is associated with changes in plasma von Willebrand factor in older humans

Modulation of circulating purines and pyrimidines by physical exercise in the horse

Effect of brief maximal exercise on circulating levels of interleukin-12

Exercise-induced changes in circulating levels of transforming growth factor-β-1 in humans: methodological considerations

Similar Documents

Exercise-induced changes in circulating levels of transforming growth factor-β-1 in humans: methodological considerations

Circulating transforming growth factor β1 (TGFβ1) is elevated by extensive exercise

Exercise-induced shear stress is associated with changes in plasma von Willebrand factor in older humans

Exercise-induced changes in blood levels of α-tocopherol

HGF suppresses the production of collagen type III and α-SMA induced by TGF-β1 in healing fibroblasts

HGF inhibits TGF-β1-induced myofibroblast differentiation and ECM deposition via MMP-2 in Achilles tendon in rat

Exercise-induced shear stress is associated with changes in plasma von Willebrand factor in older humans

Modulation of circulating purines and pyrimidines by physical exercise in the horse

Effect of brief maximal exercise on circulating levels of interleukin-12

Exercise-induced changes in circulating levels of transforming growth factor-β-1 in humans: methodological considerations