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| Content Provider | Springer Nature Link |
|---|---|
| Author | Olbrich, Laura Foehring, Daniel Happel, Patrick Brand Saberi, Beate Theiss, Carsten |
| Copyright Year | 2012 |
| Abstract | The neuronal growth cone plays a crucial role in the development of the nervous system. This highly motile structure leads the axon to its final destination by translating guidance cues into cytoskeletal rearrangements. Recently, vascular endothelial growth factor (VEGF), which is essential for angiogenesis and vascular sprouting, has been found to exert a trophic activity also on neurons, leading to an increased axonal outgrowth, similar to the well-known nerve growth factor (NGF). The neurotrophic properties of VEGF are likely to be promoted via the VEGF receptor 2 (VEGFR-2) and neuropilin-1 (NRP-1). In the long term, VEGF attracts and influences the growth cone velocity and leads to growth cone enlargement. The present study focuses on immediate VEGF effects using RFP-actin and GFP-NF-M microinjected chicken dorsal root ganglia for live cell imaging of the neuronal growth cone. We analyzed actin and neurofilament dynamics following VEGF and NGF treatment and compared the effects. Furthermore, key signaling pathways of VEGF were investigated by specific blocking of VEGFR-2 or NRP-1. With the aid of confocal laser scanning microscopy and stimulated emission depletion microscopy, we show for the first time that VEGF has a quick effect on the actin-cytoskeleton, since actin rearrangements were identifiable within a few minutes, leading to a dramatically increased motion. Moreover, these effects were strongly enhanced by adding both VEGF and NGF. Most notably, the effects were inhibited by blocking VEGFR-2, therefore we propose that the immediate effects of VEGF on the actin-cytoskeleton are mediated through VEGFR-2. |
| Starting Page | 431 |
| Ending Page | 445 |
| Page Count | 15 |
| File Format | |
| ISSN | 09486143 |
| Journal | Histochemistry and Cell Biology |
| Volume Number | 139 |
| Issue Number | 3 |
| e-ISSN | 1432119X |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-10-04 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Confocal laser scanning microscopy Actin VEGF NGF STED microscopy Biomedicine general Cell Biology Biochemistry Developmental Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Histology Molecular Biology Medical Laboratory Technology |
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