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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhao, Boqiang Ito, Kunitoshi Iyengar, Prasanna Vasudevan Hirose, Shigehisa Nakamura, buhiro |
| Copyright Year | 2012 |
| Abstract | Spermatogenesis is a highly complicated metamorphosis process of male germ cells. Recent studies have provided evidence that the ubiquitin–proteasome system plays an important role in sperm head shaping, but the underlying mechanism is less understood. In this study, we localized membrane-associated RING-CH (MARCH)7, an E3 ubiquitin ligase, in rat testis. Northern blot analysis showed that March7 mRNA is expressed ubiquitously but highly in the testis and ovary. In situ hybridization of rat testis demonstrated that March7 mRNA is expressed weakly in spermatogonia and its level is gradually increased as they develop. Immunohistochemical analysis detected MARCH7 protein expression in spermiogenic cells from late round spermatids to elongated spermatids and in epididymal spermatozoa. Moreover, MARCH7 was found to be localized to the caudal end of the developing acrosome of late round and elongating spermatids, colocalizing with β-actin, a component of the acroplaxome. In addition, MARCH7 was also detected in the developing flagella and its expression levels were prominent in elongated spermatids. We also showed that MARCH7 catalyzes lysine 48 (K48)-linked ubiquitination. Immunolocalization studies revealed that K48-linked ubiquitin chains were detected in the heads of elongating spermatids and in the acrosome/acroplaxome, neck, midpiece and cytoplasmic lobes of elongated spermatids. These results suggest that MARCH7 is involved in spermiogenesis by regulating the structural and functional integrity of the head and tail of developing spermatids. |
| Starting Page | 447 |
| Ending Page | 460 |
| Page Count | 14 |
| File Format | |
| ISSN | 09486143 |
| Journal | Histochemistry and Cell Biology |
| Volume Number | 139 |
| Issue Number | 3 |
| e-ISSN | 1432119X |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-10-27 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | K48 Membrane-associated RING-CH Spermatogenesis Ubiquitination Ubiquitin–proteasome system Biomedicine general Cell Biology Biochemistry Developmental Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Histology Molecular Biology Medical Laboratory Technology |
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