NDLI: The effect of the enkephalin DADLE on transcription does not depend on opioid receptors

Content Provider	Springer Nature Link
Author	Baldelli, Beatrice Vecchio, Lorella Bottone, Maria Grazia Muzzonigro, Giovanni Biggiogera, Marco Malatesta, Manuela
Copyright Year	2006
Abstract	[D-Ala2,D-Leu5] enkephalin (DADLE) is a synthetic peptide capable of inducing a hibernation-like state in mammals in vivo and in cultured cells in vitro. The effects of DADLE seem to be due to its binding to opioid receptors; however, it inhibits the growth of LNCaP cells, devoid of opioid receptors. We have investigated the effects of DADLE on this cell line using transmission electron microscopy, immunocytochemistry and cytometry, in order to elucidate the general mechanism(s) by which this enkephalin affects cell metabolism. We demonstrated that, similar to cell lines provided with opioid receptors, in LNCaP cells DADLE induces structural modifications of cytoplasmic and nuclear constituents, as well as a decrease in transcription and proliferation. However, DADLE does not provoke an increase in apoptotic or necrotic cell fraction, and, after removing the enkephalin from the culture medium, all effects disappear. We also demonstrated that DADLE molecules enter the cytoplasm and the nucleus of LNCaP cells, mostly binding to perichromatin fibrils and dense fibrillar component, where transcription and early splicing of pre-mRNAs and pre-rRNAs occur. In conclusion, our data demonstrate that the effect of DADLE on transcription and on cultured cells does not depend on opioid receptors. DADLE can, therefore, be envisaged as an extremely promising molecule to be used for inducing a reversible hypometabolic state in various cultured cells, without provoking cell damage or death.
Starting Page	189
Ending Page	197
Page Count	9
File Format	PDF
ISSN	09486143
Journal	Histochemistry and Cell Biology
Volume Number	126
Issue Number	2
e-ISSN	1432119X
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-01-14
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DADLE Hypometabolic state Transcription Cell nucleus Electron microscopy Anatomy Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Molecular Biology Medical Laboratory Technology

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