NDLI: Ultrastructural characterization of giant endosomes induced by GTPase-deficient Rab5

Content Provider	Springer Nature Link
Author	Wegener, Catherine Sem Malerød, Lene Pedersen, Nina Marie Prodiga, Cinzia Bakke, Oddmund Stenmark, Harald Brech, Andreas
Copyright Year	2009
Abstract	The small GTPase Rab5 controls the fusogenic properties of early endosomes through GTP-dependent recruitment and activation of effector proteins. Expression of a GTPase-defective mutant, Rab5(Q79L), is known to cause formation of enlarged early endosomes. The ability of Rab5-GTP to recruit multiple effectors raises the question whether the Rab5(Q79L)-induced giant endosomes simply represent enlarged early endosomes or whether they have a more complex phenotype. In this report, we have addressed this issue by generating a HEp2 cell line with inducible expression of Rab5(Q79L) and performing ultrastructural analysis of Rab5(Q79L)-induced endosomes. We find that Rab5(Q79L) not only induces formation of enlarged early endosomes but also causes enlargement of later endocytic profiles. Most strikingly, Rab5(Q79L) causes formation of enlarged multivesicular endosomes with a large number of intraluminal vesicles, and endosomes that contain both early and late endocytic markers are frequently observed. In addition, we observe defects in the sorting of the EGF receptor and the transferrin receptor through this compartment.
Starting Page	41
Ending Page	55
Page Count	15
File Format	PDF
ISSN	09486143
Journal	Histochemistry and Cell Biology
Volume Number	133
Issue Number	1
e-ISSN	1432119X
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-10-15
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Endosome Endocytosis Membrane traffic Rab GTPase Rab5 Medicine/Public Health Anatomy
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Molecular Biology Medical Laboratory Technology

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Erratum to: Ultrastructural characterization of giant endosomes induced by GTPase-deficient Rab5

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